Putative Integrative Mobile Elements That Exploit the Xer Recombination Machinery Carrying blaIMI-Type Carbapenemase Genes in Enterobacter cloacae Complex Isolates in Singapore

Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing Enterobacter cloacae complex to determine the flanking regions of blaIMI-type genes. Phylogenetic analysis of multilocus sequence typing (MLST) targets separated the isolates into 4 clusters. The blaIMI-type genes we...

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Published inAntimicrobial agents and chemotherapy Vol. 62; no. 1
Main Authors Koh, Tse H, Rahman, Nurdyana Binte Abdul, Teo, Jeanette W. P, La, My-Van, Periaswamy, Balamurugan, Chen, Swaine L
Format Journal Article
LanguageEnglish
Published 1752 N St., N.W., Washington, DC American Society for Microbiology 01.01.2018
Subjects
Bacterial Proteins
beta-Lactamases
Drug Resistance, Bacterial
Enterobacter cloacae
Interspersed Repetitive Sequences
Mechanisms of Resistance
antimicrobial resistance
mobile genetic elements
carbapenemase
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Summary:Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing Enterobacter cloacae complex to determine the flanking regions of blaIMI-type genes. Phylogenetic analysis of multilocus sequence typing (MLST) targets separated the isolates into 4 clusters. The blaIMI-type genes were all found on Xer-dependent integrative mobile elements (IMEX). The IMEX elements of 5 isolates were similar to those described in Canada, while the remainder were novel. Five isolates had IMEX elements lacking a resolvase and recombinase.
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Present address: My-Van La, Department of Laboratory Medicine, Changi General Hospital, Singapore.
Citation Koh TH, Rahman NBA, Teo JWP, La M-V, Periaswamy B, Chen SL. 2018. Putative integrative mobile elements that exploit the Xer recombination machinery carrying blaIMI-type carbapenemase genes in Enterobacter cloacae complex isolates in Singapore. Antimicrob Agents Chemother 62:e01542-17. https://doi.org/10.1128/AAC.01542-17.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.01542-17