Fast and Robust Method To Determine Phenoyl and Acetyl Esters of Polysaccharides by Quantitative 1H NMR

• Published in: Journal of agricultural and food chemistry Vol. 61; no. 26; pp. 6282 - 6287
• Main Authors: Neumüller, Klaus G, Carvalho de Souza, Adriana, Van Rijn, Joep, Appeldoorn, Maaike M, Streekstra, Hugo, Schols, Henk A, Gruppen, Harry
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 03.07.2013
• Subjects: Acetylation; acids; Animal Feed - analysis; Biological and medical sciences; Cell Wall - chemistry; Cell Wall - metabolism; Crops, Agricultural - chemistry; Crops, Agricultural - metabolism; Esters; extracts; Food Analysis - methods; Food industries; Fundamental and applied biological sciences. Psychology; Netherlands; nmr; nuclear magnetic resonance spectroscopy; Phenols - analysis; Phenols - chemistry; polysaccharides; Polysaccharides - analysis; Polysaccharides - chemistry; Polysaccharides - metabolism; spectroscopy; Netherlands; polysaccharides; NMR; acetyl ester; phenoyl ester; Ester; Method; Polysaccharide
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf401393c

The acetyl (AcE), feruloyl (FE), and p-coumaroyl (pCE) ester contents of different cereal and grass polysaccharides were determined by a quantitative 1H NMR-based method. The repeatability and the robustness of the method were demonstrated by analyzing different plant polysaccharide preparations. Good sensitivity and selectivity for AcE, FE, and pCE were observed. Moreover, an optimized and easy sample preparation allowed for simultaneous quantification of AcE, FE, and pCE. The method is suitable for high-throughput analysis, and it is a good alternative for currently used analytical procedures. A comparison of the method presented to a conventional HPLC-based method showed that the results obtained are in good agreement, whereas the combination of the optimized sample preparation and analysis by the 1H NMR-based methodology results in significantly reduced analysis time.