Near-Infrared Fluorescent Endoscopic Image-Guided Photothermal Ablation Therapy of Colorectal Cancer Using Dual-Modal Gold Nanorods Targeting Tumor-Infiltrating Innate Immune Cells in a Transgenic TS4 CRE/APC loxΔ468 Mouse Model

• Published in: ACS applied materials & interfaces Vol. 11; no. 24; pp. 21353 - 21359
• Main Authors: Gournaris, Elias, Park, Wooram, Cho, Soojeong, Bentrem, David J, Larson, Andrew C, Kim, Dong-Hyun
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.06.2019
• Subjects: Ablation Techniques - methods; Animals; Cell Line, Tumor; Colorectal Neoplasms - therapy; Disease Models, Animal; Gold - chemistry; Immunity, Innate - physiology; Mice; Mice, Transgenic; Nanotubes - chemistry; Phototherapy - methods; Spectroscopy, Near-Infrared; interventional oncology; nanomedicine; endoscopic image-guided therapy; colon cancer; photothermal therapy
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.9b04186

Colorectal cancer (CRC) is diagnosed with colonoscopy and treated with focal therapies. CRC is a good candidate for nanoparticle-mediated photothermal ablation (PTA) therapy. Herein, we developed a near-infrared fluorescent (NIRF) endoscopic image-guided PTA approach using a nanoparticle capable of simultaneously diagnosing and treating CRC. Dual-modal NIR heating and fluorescent gold nanorods (dual-modal GNRs) were synthesized by conjugation of GNRs to an NIRF probe. To validate the translational potential of our approach, a well-characterized transgenic TS4 CRE/APC loxΔ468 colon cancer mouse model was used to carry out NIRF image-guided PTA using our dual-modal GNRs under clinically relevant conditions. Intravenously infused dual-modal GNRs were effectively targeted at colon polyps by immunogenic capturing of the GNRs within tumor-infiltrating innate immune cells. NIRF endoscopic image-guided PTA using the GNRs permitted successful detection and ablation of inflammatory colon polyps. NIRF endoscopy image-guided PTA using dual-modal GNRs can be utilized for diagnosis and treatment of CRC and various inflammatory diseases.