Model Simulation of BSA Partitioning in Recycling Aqueous Two-Phase Systems Composed of Light-Sensitive Polymer PNBC-Dextran

• Published in: Journal of chemical and engineering data Vol. 53; no. 12; pp. 2755 - 2757
• Main Authors: Shao, Zhihua, Cao, Xuejun
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 11.12.2008
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; Proteins; Prediction; Partition coefficient; Serum albumin; Serum protein; Empirical model; Modeling; Norbornadiene derivative polymer; Dextran; Separation method; Biphasic system; Light sensitive polymer; Thermodynamic model; Simulation; Aqueous medium; Recycling
• ISSN: 0021-9568; 1520-5134
• DOI: 10.1021/je800313k

Aqueous two-phase systems (ATPS) are potential industrial techniques in the separation process of biomacromolecules, antibiotics, amino acids, and organic acids, etc. Partition behavior prediction of biomolecules in ATPS is an important problem. This paper applies the empirical protein partition model and the Florry−Huggins partition model to the experimental data of light-sensitive polymer PNBC-dextran ATPS developed by our laboratory. It has been found that the Flory−Huggins partition model could comply with the experimental results, with an average relative error of 1.82 % and maximum relative error of 3.03 %.