Point-of-Care Testing of Benzene Metabolite S‑Phenylmercapturic Acid Using Salt-Induced Phase Separation Combined with Nanoparticle-Based Surface-Enhanced Raman Spectroscopy
Benzene, a known toxic, carcinogenic, and pervasively present environmental pollutant, necessitates effective monitoring strategies to assess human exposure. S-Phenylmercapturic acid (S-PMA) is a specific biomarker for benzene exposure. This study presents a point-of-care testing (POCT) assay for th...
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Published in | ACS applied nano materials Vol. 7; no. 14; pp. 16237 - 16244 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
American Chemical Society
26.07.2024
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Subjects | |
Online Access | Get full text |
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Summary: | Benzene, a known toxic, carcinogenic, and pervasively present environmental pollutant, necessitates effective monitoring strategies to assess human exposure. S-Phenylmercapturic acid (S-PMA) is a specific biomarker for benzene exposure. This study presents a point-of-care testing (POCT) assay for the detection of S-PMA in human urine, integrating salt-induced phase separation (SIPS) with surface-enhanced Raman spectroscopy (SERS). S-PMA in human urine was first effectively separated using SIPS and then detected using a portable Raman spectrometer employing Au colloids as the SERS substrate. The POCT assay exhibited a clear correlation between the signal intensity and logarithmic concentration of S-PMA (0–5 ppm), demonstrating satisfactory recovery and reproducibility. Moreover, high-throughput fingerprint identification and quantitative analysis of S-PMA in urine were achieved within 10 min, with a detection limit as low as 1.06 ppb, by performing SIPS-SERS on 96-well plates. This POCT method holds promise for personal exposure assessment and may potentially aid in the prevention of benzene-induced diseases. |
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ISSN: | 2574-0970 2574-0970 |
DOI: | 10.1021/acsanm.4c02165 |