1H NMR-Based Protocol for the Detection of Adulterations of Refined Olive Oil with Refined Hazelnut Oil

• Published in: Journal of agricultural and food chemistry Vol. 57; no. 24; pp. 11550 - 11556
• Main Authors: Mannina, Luisa, D’Imperio, Marco, Capitani, Donatella, Rezzi, Serge, Guillou, Claude, Mavromoustakos, Thomas, Vilchez, María Dolores Molero, Fernández, Antonio Herrera, Thomas, Freddy, Aparicio, Ramon
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 23.12.2009
• Subjects: adulterated products; Biological and medical sciences; Corylus - chemistry; detection; Dietary Fats, Unsaturated - analysis; equipment performance; Fat industries; food analysis; Food Chemistry/Biochemistry; food composition; Food Contamination; Food industries; Food Technology - methods; Food Technology - standards; fraud; Fruit - chemistry; Fundamental and applied biological sciences. Psychology; General aspects; hazelnut oil; Limit of Detection; Magnetic Resonance Spectroscopy - methods; Methods of analysis, processing and quality control, regulation, standards; Models, Statistical; new methods; nuclear magnetic resonance spectroscopy; Nuts - chemistry; Olea - chemistry; Olive Oil; Plant Oils - chemistry; product authenticity; Reproducibility of Results; olive oil; NMR; Hazelnut oil; statistical analysis; Bass; Edible fish; Nuts; Statistical analysis; Hazelnut; Vegetable oil; Olive oil; Edible oil; Analysis method; Control method; Refined oil; Detection; Adulteration
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf902426b

A 1H NMR analytical protocol for the detection of refined hazelnut oils in admixtures with refined olive oils is reported according to ISO format. The main purpose of this research activity is to suggest a novel analytical methodology easily usable by operators with a basic knowledge of NMR spectroscopy. The protocol, developed on 92 oil samples of different origins within the European MEDEO project, is based on 1H NMR measurements combined with a suitable statistical analysis. It was developed using a 600 MHz instrument and was tested by two independent laboratories on 600 MHz spectrometers, allowing detection down to 10% adulteration of olive oils with refined hazelnut oils. Finally, the potential and limitations of the protocol applied on spectrometers operating at different magnetic fields, that is, at the proton frequencies of 500 and 400 MHz, were investigated.