Structural and Dynamic Parameters Obtained from 17O NMR, EPR, and NMRD Studies of Monomeric and Dimeric Gd3+ Complexes of Interest in Magnetic Resonance Imaging:  An Integrated and Theoretically Self-Consistent Approach1

• Published in: Journal of the American Chemical Society Vol. 118; no. 39; pp. 9333 - 9346
• Main Authors: Powell, D. Hugh, Dhubhghaill, Orla M. Ni, Pubanz, Dirk, Helm, Lothar, Lebedev, Yakob S, Schlaepfer, Willi, Merbach, André E
• Format: Journal Article
• Language: English; Japanese
• Published: American Chemical Society 02.10.1996
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja961743g

We present the results of new and previously published 17O NMR, EPR, and NMRD studies of aqueous solutions of the Gd3+ octaaqua ion and the commercial MRI contrast agents [Gd(DTPA)(H2O)]2- (MAGNEVIST, Schering AG, DTPA = 1,1,4,7,7-pentakis(carboxymethyl)-1,4,7-triazaheptane), [Gd(DTPA-BMA)(H2O)] (OMNISCAN, Sanofi Nycomed, DTPA-BMA = 1,7-bis[(N-methylcarbamoyl)methyl]-1,4,7-tris(carboxymethyl)-1,4,7-triazaheptane), and [Gd(DOTA)(H2O)]- (DOTAREM, Guerbet, DOTA = 1,4,7,10-tetrakis(carboxymethyl)-1,4,7,10-tetraazacyclododecane). High-field EPR measurements at different concentrations give evidence of an intermolecular dipole−dipole electronic relaxation mechanism that has not previously been described for Gd3+ complexes. For the first time, the experimental data from the three techniques for each complex have been treated using a self-consistent theoretical model in a simultaneous multiple parameter least-squares fitting procedure. The lower quality of the fits compared to separate fits of the data for each of the three techniques shows that the increase in the number of adjustable parameters is outweighed by the increased constraint on the fits. The parameters governing the relaxivity of the complexes are thus determined with greater confidence than previously possible. The same approach was used to study two dimeric Gd3+ complexes [pip{Gd(DO3A)(H2O)}2] and [bisoxa{Gd(DO3A)(H2O)}2] (pip(DO3A)2 = bis(1,4-(1-(carboxymethyl)-1,4,7,10-tetraaza-4,7,10-tris(carboxymethyl)-1-cyclododecyl-1,4-diazacyclohexane, bisoxa(DO3A)2 = bis(1,4-(1-(carboxymethyl)-1,4,7,10-tetraaza-4,7,10-tris(carboxymethyl)-1-cyclododecyl))-1,10-diaza-3,6-dioxadecane) that are being developed as potential second-generation MRI contrast agents. These dimeric complexes are expected to have higher relaxivities than the monomeric contrast agents, due to their longer rotational correlation times. The results of this study show that further relaxivity gain for these complexes will be hindered by the slow rate of water exchange on the complexes. High-field EPR measurements suggest that there is a previously unrecorded intramolecular dipole−dipole mechanism of electronic relaxation, but that this additional contribution to electronic relaxation is of minor importance compared to the limiting effect of water exchange rates in the determination of proton relaxivity in MRI applications.