Enzymatic Hydrolysis of Chemosynthesized Atactic Poly(3-hydroxybutyrate) by Poly(3-hydroxyalkanoate) Depolymerase from Acidovorax Sp. TP4 and Ralstonia p ickettii T1

• Published in: Biomacromolecules Vol. 3; no. 5; pp. 894 - 898
• Main Authors: Wang, Yi, Inagawa, Yasuhide, Osanai, Yasushi, Kasuya, Ken-ichi, Saito, Terumi, Matsumura, Shuichi, Doi, Yoshiharu, Inoue, Yoshio
• Format: Journal Article
• Language: English
• Published: American Chemical Society 09.09.2002
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm020052b

The enzymatic degradability of chemosynthesized atactic poly([R,S]-3-hydroxybutyrate) [a-P(3HB)] by two types of extracellular poly(3-hydroxyalkanoate) (PHA) depolymerases purified from Ralstonia pickettii T1 (PhaZ ral) and Acidovorax Sp. TP4 (PhaZ aci), defined respectively as PHA depolymerase types I and II according to the position of the lipase box in the catalytic domain, were studied. The enzymatic degradation of a-P(3HB) by PhaZ aci depolymerase was confirmed from the results of weight loss and the scanning electron micrographs. The degradation products were characterized by one- and two-dimension 1H NMR spectroscopy. It was found that a-P(3HB) could be degraded into monomer, dimer, and trimer by PhaZ aci depolymerase at temperatures ranging from 4 to 20 °C, while a-P(3HB) could hardly be hydrolyzed by PhaZ ral depolymerase in the same temperature range. These results suggested that the chemosynthesized a-P(3HB) could be degraded in the pure state by natural PHA depolymerase.