Unprecedented Sensitivity in a Probe for Monitoring CathepsinB: Chemiluminescence Microscopy Cell-Imaging of a Natively Expressed Enzyme

• Published in: Angewandte Chemie International Edition Vol. 56; no. 49; pp. 15633 - 15638
• Main Authors: Roth-Konforti, Michal E., Bauer, Christoph R., Shabat, Doron
• Format: Journal Article
• Language: English
• Published: WEINHEIM Wiley 04.12.2017
• Subjects: Chemistry; Chemistry, Multidisciplinary; Physical Sciences; Science & Technology; CATALYZED CHEMILUMINESCENCE; dioxetanes; SUBSTITUTED DIOXETANE; cathepsin B; chemiluminescence; DOXORUBICIN; RELEASE; MODEL; 1,2-DIOXETANES; live-cell imaging; molecular probes; PROTEASE ACTIVITY; BIOLUMINESCENCE

Until recently, chemiluminescence cell images could only be obtained using luciferase-activated probes. Moreover, chemiluminescence microscopy cell-imaging has not been demonstrated for natively expressed enzymes like cathepsin B. Herein, we describe the design, synthesis, and evaluation of the firs...