花生蛋白突变体籽粒不同发育时期转录组分析
S565.2; 花生是优质的食用蛋白资源,在食品工业中有广泛的应用.本研究通过对潍花8号进行EMS诱变获得蛋白质含量显著增加的突变体,并对潍花8号突变体及其野生型开花后20天、40天和60天的籽粒分别进行转录组测序,构建野生型(P05-20、P05-40、P05-60)和突变体(P06-20、P06-40、P06-60)样品文库.将野生型与突变体3个不同发育时期测序结果进行比对,分别获得2936、3329、2849个差异表达基因,上调的差异表达基因为1599、2471、707个,下调的差异表达基因为1337、858、2142个.对差异基因进行KEGG富集分析,分别得到26、23、31条具有显著...
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Published in | 中国油料作物学报 Vol. 44; no. 5; pp. 1018 - 1029 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | Chinese |
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吉林农业大学农学院,吉林长春,130118%潍坊市农业农村局,山东潍坊,261061
2022
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Abstract | S565.2; 花生是优质的食用蛋白资源,在食品工业中有广泛的应用.本研究通过对潍花8号进行EMS诱变获得蛋白质含量显著增加的突变体,并对潍花8号突变体及其野生型开花后20天、40天和60天的籽粒分别进行转录组测序,构建野生型(P05-20、P05-40、P05-60)和突变体(P06-20、P06-40、P06-60)样品文库.将野生型与突变体3个不同发育时期测序结果进行比对,分别获得2936、3329、2849个差异表达基因,上调的差异表达基因为1599、2471、707个,下调的差异表达基因为1337、858、2142个.对差异基因进行KEGG富集分析,分别得到26、23、31条具有显著差异的代谢通路,其中与蛋白质合成相关的通路有糖酵解/糖异生、脂肪酸生物合成、丙酮酸代谢,并在其中筛选到15个与蛋白质合成相关的候选基因.该转录组测序结果有利于揭示花生籽仁中蛋白质合成调控的相关基因,为更好地理解油料作物蛋白质合成机制提供了理论依据. |
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AbstractList | S565.2; 花生是优质的食用蛋白资源,在食品工业中有广泛的应用.本研究通过对潍花8号进行EMS诱变获得蛋白质含量显著增加的突变体,并对潍花8号突变体及其野生型开花后20天、40天和60天的籽粒分别进行转录组测序,构建野生型(P05-20、P05-40、P05-60)和突变体(P06-20、P06-40、P06-60)样品文库.将野生型与突变体3个不同发育时期测序结果进行比对,分别获得2936、3329、2849个差异表达基因,上调的差异表达基因为1599、2471、707个,下调的差异表达基因为1337、858、2142个.对差异基因进行KEGG富集分析,分别得到26、23、31条具有显著差异的代谢通路,其中与蛋白质合成相关的通路有糖酵解/糖异生、脂肪酸生物合成、丙酮酸代谢,并在其中筛选到15个与蛋白质合成相关的候选基因.该转录组测序结果有利于揭示花生籽仁中蛋白质合成调控的相关基因,为更好地理解油料作物蛋白质合成机制提供了理论依据. |
Author | 张君 谭乐增 姚丹 吴秋霖 邢永祎 梁嘉宁 韩笑 杨松楠 李雪莹 陈亮宇 |
AuthorAffiliation | 吉林农业大学农学院,吉林长春,130118%潍坊市农业农村局,山东潍坊,261061 |
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Author_FL | LIANG Jia-ning LI Xue-ying CHEN Liang-yu XING Yong-yi ZHANG Jun YAO Dan HAN Xiao TAN Le-zeng YANG Song-nan WU Qiu-lin |
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Title | 花生蛋白突变体籽粒不同发育时期转录组分析 |
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