旱柳CCD基因家族鉴定及其对盐涝胁迫的表达分析

S792.12; [目的]类胡萝卜素裂解双加氧酶(CCD)催化多种类胡萝卜素裂解成更小的分子,这一过程对于植物的生长、发育和对非生物胁迫的响应至关重要.深入理解类胡萝卜素的生物合成和降解机制,对精确调控其在植物体内的含量至关重要.[方法]选取中国本土品种旱柳(Salix matsudana)为试验材料,利用旱柳的全基因组数据库采用构建系统进化树、分析顺式作用元件、探究保守基序和基因结构、分析共线性、构建蛋白互作网络、GO注释分析,以及转录组分析等方法,对旱柳CCD家族成员(SmCCD)进行鉴定分析.[结果]从旱柳基因组中成功鉴定出17个CCD基因,这些SmCCD成员被归类为两个亚家族:SmCC...

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Published in	西南农业学报 Vol. 37; no. 10; pp. 2157 - 2168
Main Authors	张兴月, 陈金鑫, 王欣, 刘国元, 余春梅, 张健, 魏辉
Format	Journal Article
Language	Chinese
Published	南通市观赏植物遗传育种重点实验室,江苏南通 226019 2024 南通大学生命科学学院,江苏南通 226019
Subjects	CCD基因 Expression analysis 表达分析 Salix matsudana 共线性分析 CCD gene Collinearity analysis 旱柳
Online Access	Get full text
ISSN	1001-4829
DOI	10.16213/j.cnki.scjas.2024.10.003

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Abstract	S792.12; [目的]类胡萝卜素裂解双加氧酶(CCD)催化多种类胡萝卜素裂解成更小的分子,这一过程对于植物的生长、发育和对非生物胁迫的响应至关重要.深入理解类胡萝卜素的生物合成和降解机制,对精确调控其在植物体内的含量至关重要.[方法]选取中国本土品种旱柳(Salix matsudana)为试验材料,利用旱柳的全基因组数据库采用构建系统进化树、分析顺式作用元件、探究保守基序和基因结构、分析共线性、构建蛋白互作网络、GO注释分析,以及转录组分析等方法,对旱柳CCD家族成员(SmCCD)进行鉴定分析.[结果]从旱柳基因组中成功鉴定出17个CCD基因,这些SmCCD成员被归类为两个亚家族:SmCCD和SmNCED.SmCCD亚家族进一步细分为SmCCD1、SmCCD4、SmCCD7和SmCCD8等类别.共线性分析揭示片段重复是导致SmCCD基因扩增的关键因素.对杨树、拟南芥、水稻与旱柳之间的基因共线性进行分析,发现杨树与旱柳和拟南芥之间的基因对数量多于旱柳与水稻之间的基因对,这表明旱柳与杨树等双子叶植物之间存在较近的进化关系.转录组分析结果表明,SmCCD基因在旱柳遭遇盐胁迫和淹水胁迫时表达水平显著变化,这暗示该基因在旱柳适应环境压力中可能发挥关键作用.[结论]揭示SmnCCD基因家族的多样性和在环境胁迫下的表达模式,为理解旱柳的适应性和进化关系提供新的分子证据.这有助于指导旱柳的育种和生态恢复工作,以应对环境变化的挑战.
AbstractList	S792.12; [目的]类胡萝卜素裂解双加氧酶(CCD)催化多种类胡萝卜素裂解成更小的分子,这一过程对于植物的生长、发育和对非生物胁迫的响应至关重要.深入理解类胡萝卜素的生物合成和降解机制,对精确调控其在植物体内的含量至关重要.[方法]选取中国本土品种旱柳(Salix matsudana)为试验材料,利用旱柳的全基因组数据库采用构建系统进化树、分析顺式作用元件、探究保守基序和基因结构、分析共线性、构建蛋白互作网络、GO注释分析,以及转录组分析等方法,对旱柳CCD家族成员(SmCCD)进行鉴定分析.[结果]从旱柳基因组中成功鉴定出17个CCD基因,这些SmCCD成员被归类为两个亚家族:SmCCD和SmNCED.SmCCD亚家族进一步细分为SmCCD1、SmCCD4、SmCCD7和SmCCD8等类别.共线性分析揭示片段重复是导致SmCCD基因扩增的关键因素.对杨树、拟南芥、水稻与旱柳之间的基因共线性进行分析,发现杨树与旱柳和拟南芥之间的基因对数量多于旱柳与水稻之间的基因对,这表明旱柳与杨树等双子叶植物之间存在较近的进化关系.转录组分析结果表明,SmCCD基因在旱柳遭遇盐胁迫和淹水胁迫时表达水平显著变化,这暗示该基因在旱柳适应环境压力中可能发挥关键作用.[结论]揭示SmnCCD基因家族的多样性和在环境胁迫下的表达模式,为理解旱柳的适应性和进化关系提供新的分子证据.这有助于指导旱柳的育种和生态恢复工作,以应对环境变化的挑战.
Abstract_FL	[Objective]Carotenoid cleavage dioxygenases(CCDs)catalyze the breakdown of various carotenoids into smaller molecules,a process essential for plant growth,development and response to abiotic stresses.A thorough understanding of the biosynthesis and degradation mechanisms of carotenoids is crucial for precisely regulating their levels within plants.[Method]We selected the indigenous Chinese variety of Salix matsudana as the experimental material and utilized its whole-genome database to identify and analyzed members of the SmCCD fami-ly.The research included constructing a phylogenetic tree,analyzing cis-acting elements,exploring conserved motifs and gene structures,con-ducting collinearity analysis,building protein interaction networks,performing Gene Ontology(GO)annotation analysis and transcriptome a-nalysis.[Result]The study successfully identified 17 CCD genes from the S.matsudana genome and conducted an in-depth analysis of their characteristics and expression patterns.These SmCCD members were classified into two subfamilies:SmCCD and SmNCED.The SmCCD sub-family was further divided into categories such as SmCCD1,SmCCD4,SmCCD7 and SmCCD8.Collinearity analysis revealed that segmental duplication was a key factor leading to the amplification of SmCCD genes.By comparing the gene collinearity between willow,Arabidopsis,rice and poplar,we found a higher number of gene pairs among willow,poplar and Arabidopsis than between willow and rice,supporting the closer evolutionary relationship between willow and other dicotyledonous plants such as poplar.Additionally,transcriptome analysis indicated signifi-cant changes in the expression levels of SmCCD genes in S.matsudana under salt and waterlogging stresses,suggesting their potential key role in the plant's adaptation to environmental stress.[Conclusion]The study reveals the diversity of the SmCCD gene family and their expression patterns under environmental stress,providing new molecular evidence for understanding the adaptability and evolutionary relationships of wil-low.These findings are instrumental in guiding the breeding and ecological restoration of willow to address the challenges of environmental change.
Author	刘国元陈金鑫王欣魏辉张兴月张健余春梅
AuthorAffiliation	南通大学生命科学学院,江苏南通 226019;南通市观赏植物遗传育种重点实验室,江苏南通 226019
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Author_FL	LIU Guo-yuan ZHANG Jian YU Chun-mei CHEN Jin-xin WEI Hui WANG Xin ZHANG Xing-yue
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