脉络宁注射液干预挤压伤综合征模型猪线粒体自噬及PINK1/Parkin通路的变化
R459.9%R318%R846+.1; 背景:挤压伤综合征常引起实质性脏器损害,且其病情发展较快、死亡率高,探究其病理机制,缓解缺血再灌注损伤,可改善挤压伤病理症状.目的:模拟体内生理环境体外灌注脉络宁注射液对挤压伤综合征模型猪线粒体自噬及PINK1/Parkin通路的影响.方法:将巴马小型猪随机分为4组:对照组、模型组、灌注组、脉络宁组,除对照组外,其余3组制备挤压伤模型,灌注组解除挤压后先进行体外循环灌注再恢复自体血供,灌注过程中以50 mL/h的速度滴入生理盐水50 mL,脉络宁组在灌注过程中以50 mL/h的速度滴入脉络宁注射液50 mL,自体供血恢复6 h后,用全自动生化分析仪检测...
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Published in | 中国组织工程研究 Vol. 25; no. 17; pp. 2676 - 2680 |
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Main Authors | , , , |
Format | Journal Article |
Language | Chinese |
Published |
首都医科大学附属北京世纪坛医院骨科,北京市 100038
2021
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Subjects | |
Online Access | Get full text |
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Abstract | R459.9%R318%R846+.1; 背景:挤压伤综合征常引起实质性脏器损害,且其病情发展较快、死亡率高,探究其病理机制,缓解缺血再灌注损伤,可改善挤压伤病理症状.目的:模拟体内生理环境体外灌注脉络宁注射液对挤压伤综合征模型猪线粒体自噬及PINK1/Parkin通路的影响.方法:将巴马小型猪随机分为4组:对照组、模型组、灌注组、脉络宁组,除对照组外,其余3组制备挤压伤模型,灌注组解除挤压后先进行体外循环灌注再恢复自体血供,灌注过程中以50 mL/h的速度滴入生理盐水50 mL,脉络宁组在灌注过程中以50 mL/h的速度滴入脉络宁注射液50 mL,自体供血恢复6 h后,用全自动生化分析仪检测血清肌酐、尿素氮、肌酸激酶、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、钾离子生化指标;ELISA检测血清白细胞介素1β、白细胞介素6、肿瘤坏死因子α水平;苏木精-伊红染色观察胫前肌组织病理变化;透射电镜观察胫前肌组织线粒体变化及自噬小体数量;Western blot检测胫前肌组织LC3-Ⅰ和LC3-Ⅱ、PINK1、Parkin蛋白表达.结果与结论:①与对照组相比,模型组、灌注组小猪胫前肌组织肌丝紊乱,炎症细胞浸润,血清各项生化指标和炎症指标均显著升高(P均<0.001),胫前肌组织自噬小体数目、LC3-Ⅱ/LC3-Ⅰ、PINK1、Parkin蛋白表达显著降低(P均<0.05);②与模型组、灌注组相比,脉络宁组胫前肌组织上述病理症状明显减轻,血清各项生化指标和炎症指标均显著降低(P均<0.001),胫前肌组织自噬小体数目、LC3-Ⅱ/LC3-Ⅰ、PINK1、Parkin蛋白表达显著增加(P均<0.05);③结果 表明,脉络宁注射液可能通过PINK1/Parkin通路激活线粒体自噬,改善挤压伤病理症状. |
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AbstractList | R459.9%R318%R846+.1; 背景:挤压伤综合征常引起实质性脏器损害,且其病情发展较快、死亡率高,探究其病理机制,缓解缺血再灌注损伤,可改善挤压伤病理症状.目的:模拟体内生理环境体外灌注脉络宁注射液对挤压伤综合征模型猪线粒体自噬及PINK1/Parkin通路的影响.方法:将巴马小型猪随机分为4组:对照组、模型组、灌注组、脉络宁组,除对照组外,其余3组制备挤压伤模型,灌注组解除挤压后先进行体外循环灌注再恢复自体血供,灌注过程中以50 mL/h的速度滴入生理盐水50 mL,脉络宁组在灌注过程中以50 mL/h的速度滴入脉络宁注射液50 mL,自体供血恢复6 h后,用全自动生化分析仪检测血清肌酐、尿素氮、肌酸激酶、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、钾离子生化指标;ELISA检测血清白细胞介素1β、白细胞介素6、肿瘤坏死因子α水平;苏木精-伊红染色观察胫前肌组织病理变化;透射电镜观察胫前肌组织线粒体变化及自噬小体数量;Western blot检测胫前肌组织LC3-Ⅰ和LC3-Ⅱ、PINK1、Parkin蛋白表达.结果与结论:①与对照组相比,模型组、灌注组小猪胫前肌组织肌丝紊乱,炎症细胞浸润,血清各项生化指标和炎症指标均显著升高(P均<0.001),胫前肌组织自噬小体数目、LC3-Ⅱ/LC3-Ⅰ、PINK1、Parkin蛋白表达显著降低(P均<0.05);②与模型组、灌注组相比,脉络宁组胫前肌组织上述病理症状明显减轻,血清各项生化指标和炎症指标均显著降低(P均<0.001),胫前肌组织自噬小体数目、LC3-Ⅱ/LC3-Ⅰ、PINK1、Parkin蛋白表达显著增加(P均<0.05);③结果 表明,脉络宁注射液可能通过PINK1/Parkin通路激活线粒体自噬,改善挤压伤病理症状. |
Author | 高磊 李天博 王江宁 陈天贵 |
AuthorAffiliation | 首都医科大学附属北京世纪坛医院骨科,北京市 100038 |
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Author_FL | Li Tianbo Wang Jiangning Chen Tiangui Gao Lei |
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DocumentTitle_FL | Effects of Mailuoning Injection on mitochondrial autophagy and PINK1/Parkin pathway in a pig model of crush injury syndrome |
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Keywords | 挤压伤综合征 自噬 胫前肌 脉络宁注射液 通路 线粒体 体外循环灌注 |
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Title | 脉络宁注射液干预挤压伤综合征模型猪线粒体自噬及PINK1/Parkin通路的变化 |
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