微波水解-液相色谱串联质谱法测定16种氨基酸的含量

目的 建立微波水解-液相色谱串联质谱法(liquid chromatography tandem mass spectrometry,LC-MS)测定保健食品中16种氨基酸的含量.方法 取适量样品,加入6 mol/L盐酸溶液15 mL,加入适量苯酚,于150℃微波消解4 h,冷却稀释至80℃减压干燥1 h.样品经0.1％甲酸溶解后经Agilent-C18柱分离,以乙腈-10 mmol/L乙酸铵溶液为流动相进行梯度洗脱,以串联质谱的多反应监测模式进行检测,外标法定量.结果 方法的线性范围0.01～0.05 nmol/μL;平均回收率为95.6％～100.7％,相对标准偏差(relative st...

Full description

Saved in:
Bibliographic Details
Published in食品安全质量检测学报 Vol. 9; no. 17; pp. 4729 - 4733
Main Authors 陈彩云, 黎小兰, 张喜金, 蔡伟江
Format Journal Article
LanguageChinese
Published 汤臣倍健股份有限公司,珠海,519040 2018
Subjects
微波水解
液相色谱-串联质谱法
氨基酸
保健品
Online AccessGet full text

Cover

Abstract 目的 建立微波水解-液相色谱串联质谱法(liquid chromatography tandem mass spectrometry,LC-MS)测定保健食品中16种氨基酸的含量.方法 取适量样品,加入6 mol/L盐酸溶液15 mL,加入适量苯酚,于150℃微波消解4 h,冷却稀释至80℃减压干燥1 h.样品经0.1％甲酸溶解后经Agilent-C18柱分离,以乙腈-10 mmol/L乙酸铵溶液为流动相进行梯度洗脱,以串联质谱的多反应监测模式进行检测,外标法定量.结果 方法的线性范围0.01～0.05 nmol/μL;平均回收率为95.6％～100.7％,相对标准偏差(relative standard deviation,RSD)小于3.0％.结论 该方法分析速度快、灵敏度高、准确和重复性好,与现有技术对比,该方法通过微波技术辅助水解,缩短了样品的前处理时间,可适用于保健食品中16种氨基酸的快速检测.
AbstractList 目的 建立微波水解-液相色谱串联质谱法(liquid chromatography tandem mass spectrometry,LC-MS)测定保健食品中16种氨基酸的含量.方法 取适量样品,加入6 mol/L盐酸溶液15 mL,加入适量苯酚,于150℃微波消解4 h,冷却稀释至80℃减压干燥1 h.样品经0.1％甲酸溶解后经Agilent-C18柱分离,以乙腈-10 mmol/L乙酸铵溶液为流动相进行梯度洗脱,以串联质谱的多反应监测模式进行检测,外标法定量.结果 方法的线性范围0.01～0.05 nmol/μL;平均回收率为95.6％～100.7％,相对标准偏差(relative standard deviation,RSD)小于3.0％.结论 该方法分析速度快、灵敏度高、准确和重复性好,与现有技术对比,该方法通过微波技术辅助水解,缩短了样品的前处理时间,可适用于保健食品中16种氨基酸的快速检测.
Author 陈彩云
黎小兰
张喜金
蔡伟江
AuthorAffiliation 汤臣倍健股份有限公司,珠海,519040
AuthorAffiliation_xml – name: 汤臣倍健股份有限公司,珠海,519040
Author_FL CAI Wei-Jiang
CHEN Cai-Yun
LI Xiao-Lan
ZHANG Xi-Jin
Author_FL_xml – sequence: 1
  fullname: CHEN Cai-Yun
– sequence: 2
  fullname: LI Xiao-Lan
– sequence: 3
  fullname: ZHANG Xi-Jin
– sequence: 4
  fullname: CAI Wei-Jiang
Author_xml – sequence: 1
  fullname: 陈彩云
– sequence: 2
  fullname: 黎小兰
– sequence: 3
  fullname: 张喜金
– sequence: 4
  fullname: 蔡伟江
BookMark eNrjYmDJy89LZWHgNDKwNNU1MLYw5GDgLS7OTDIwMDc0sjQzMuNkcH26b92zzYuebdjyYvli3WfbNj2fveNF56YXGzY-2bHpReOUF1tWANnPNk99trX76bpZhmbPl_c-27Di6fxdL1t3PJ_V8nTC6pft_TwMrGmJOcWpvFCam0HbzTXE2UO3PDEvLTEvPT4rv7QoDygTX1yQWFiVk5WcVWxkYGhhaG5gYmxMmmoAP3Ratw
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
Discipline Diet & Clinical Nutrition
DocumentTitle_FL Determination of 16 kinds of amino acids by microwave hydrolysis-liquid chromatography tandem mass spectrometry
EndPage 4733
ExternalDocumentID spaqzljcjs201817043
GroupedDBID -02
2B.
2C0
4A8
92H
92I
93N
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CDRFL
C~G
DYU
OK1
OZF
PSX
TCJ
TGT
U1G
U5L
ID FETCH-wanfang_journals_spaqzljcjs2018170433
ISSN 2095-0381
IngestDate Tue Feb 13 23:46:49 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 17
Keywords 微波水解
液相色谱-串联质谱法
氨基酸
保健品
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-wanfang_journals_spaqzljcjs2018170433
ParticipantIDs wanfang_journals_spaqzljcjs201817043
PublicationCentury 2000
PublicationDate 2018
PublicationDateYYYYMMDD 2018-01-01
PublicationDate_xml – year: 2018
  text: 2018
PublicationDecade 2010
PublicationTitle 食品安全质量检测学报
PublicationTitle_FL Journal of Food Safety & Quality
PublicationYear 2018
Publisher 汤臣倍健股份有限公司,珠海,519040
Publisher_xml – name: 汤臣倍健股份有限公司,珠海,519040
SSID ssib007129626
ssib017479571
ssib051376490
ssib007891667
ssj0001257730
Score 4.2874794
Snippet 目的 建立微波水解-液相色谱串联质谱法(liquid chromatography tandem mass spectrometry,LC-MS)测定保健食品中16种氨基酸的含量.方法 取适量样品,加入6 mol/L盐酸溶液15 mL,加入适量苯酚,于150℃微波消解4 h,冷却稀释至80℃减压干燥1...
SourceID wanfang
SourceType Aggregation Database
StartPage 4729
Title 微波水解-液相色谱串联质谱法测定16种氨基酸的含量
URI https://d.wanfangdata.com.cn/periodical/spaqzljcjs201817043
Volume 9
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR3LahRBcIg5eRGfGF_sIeVFRnoePdN97N7MEjzklEA8hZ11Vg1hRbO55CYooiJ60Es8CHpIQAgxibJ48GvczeYvrKrpTSaSQxSWoaa6Xl21vV3d2w_Pm1RhHsdFS_hBlBd-3BaFn4e67bd0mDTztrgXN3m1xUwyPRffmZfzY6deVVYtrXTz263VY_eV_E9UEYdxpV2y_xDZA6GIQBjji0-MMD5PFGPIJNgMTAZZAjYCEzIgwMaQKTApmMhnFH6wLAVtwSoqU5oxiqkDyGLClxgVgGZ-lGJUhYZ1aMmABGVJPerWBkfmKBvVqSlnAPFJ0A2wBjINSrJa1G9AxVyENJaLkOvI2kJCmoh4iSwic0o9aDICKMoZVVrnJJBe5FLiqHVTYBLCKANGjr5cxKSRj220SKK5_gZ0UCWxFlTGJIIVsG4rDknwrQ5GsJ0J6LozpipFkStN6d861ykhV-pGdbbFdQ3UMly5Yf8rih_rFexZSb4wkovCkVRLNchYPTmIa6bFyFN1Bhrk_bBOAUDYjHxkU0Ribi3Ko6zK_iAUtJk-Km-4GXVeutpG00pPFKduJqlwr-V5I38dLo60j1eXFluLy1TZIBUxHZwbi4hnJ-7OHfw0p5gXJpVMPVU4rjjMXHFcm2p5OLKQAXZcsfsDtpzulGnKN_8cVIO3yXXazc79SkY3e9Y744ZiNVO2q3Pe2OqD897E1MOiW7tZc-flLtVmRtdVXPCy_q_Nwc7nwdbucP2LP_ixvfexN3y5Pdz69ru3PXz6fri7gfBg58Pg--v-5lqQ7K2_GWxt9D_93H_e21t71n_3df_F24verUY2W5_2nVkLrqkvLxzjpuiSN9551CkuezWpm6EsZFs205xOZ8pzTAEL9JFO2qqZyAlv8iQSr5yM7Kp3muByGvCaN959slJcx8S4m9_gkP0BBnaQsQ
link.rule.ids 315,783,787,4033
linkProvider CAB International
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%BE%AE%E6%B3%A2%E6%B0%B4%E8%A7%A3-%E6%B6%B2%E7%9B%B8%E8%89%B2%E8%B0%B1%E4%B8%B2%E8%81%94%E8%B4%A8%E8%B0%B1%E6%B3%95%E6%B5%8B%E5%AE%9A16%E7%A7%8D%E6%B0%A8%E5%9F%BA%E9%85%B8%E7%9A%84%E5%90%AB%E9%87%8F&rft.jtitle=%E9%A3%9F%E5%93%81%E5%AE%89%E5%85%A8%E8%B4%A8%E9%87%8F%E6%A3%80%E6%B5%8B%E5%AD%A6%E6%8A%A5&rft.au=%E9%99%88%E5%BD%A9%E4%BA%91&rft.au=%E9%BB%8E%E5%B0%8F%E5%85%B0&rft.au=%E5%BC%A0%E5%96%9C%E9%87%91&rft.au=%E8%94%A1%E4%BC%9F%E6%B1%9F&rft.date=2018&rft.pub=%E6%B1%A4%E8%87%A3%E5%80%8D%E5%81%A5%E8%82%A1%E4%BB%BD%E6%9C%89%E9%99%90%E5%85%AC%E5%8F%B8%2C%E7%8F%A0%E6%B5%B7%2C519040&rft.issn=2095-0381&rft.volume=9&rft.issue=17&rft.spage=4729&rft.epage=4733&rft.externalDocID=spaqzljcjs201817043
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fspaqzljcjs%2Fspaqzljcjs.jpg