临床常见产ampC β-内酰胺酶菌株中染色质ampD基因突变位点的研究
Q814; 目的 了解临床常见产ampC β-内酰胺酶菌株中染色体ampD的分布及其突变位点.方法 41株临床常见产ampC β-内酰胺酶菌株(阴沟肠杆菌,肺炎克雷伯菌,鲍曼不动杆菌,铜绿假单胞菌)分离自医院感染样本,抽提其染色质DNA,PCR法扩增ampD基因并连接入pMD19-T载体后,再行PCR确定其存在.双链测序后比对染色体ampD基因的突变位点.结果 41株细菌的基因组中,使用PCR法扩增出染色体ampD基因有36株,并成功测定了其ampD基因的序列;比对后发现阴沟肠杆菌、肺炎克雷伯菌、鲍曼不动杆菌和铜绿假单胞菌的染色体ampD基因的核苷酸序列差异较大.染色质ampD易突变位点分析统...
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| Published in | 复旦学报（医学版） Vol. 36; no. 3; pp. 337 - 357 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
复旦大学附属华东医院检验科,上海,200040%浙江中医药大学附属新华医院检验科,杭州,310005
2009
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Q814; 目的 了解临床常见产ampC β-内酰胺酶菌株中染色体ampD的分布及其突变位点.方法 41株临床常见产ampC β-内酰胺酶菌株(阴沟肠杆菌,肺炎克雷伯菌,鲍曼不动杆菌,铜绿假单胞菌)分离自医院感染样本,抽提其染色质DNA,PCR法扩增ampD基因并连接入pMD19-T载体后,再行PCR确定其存在.双链测序后比对染色体ampD基因的突变位点.结果 41株细菌的基因组中,使用PCR法扩增出染色体ampD基因有36株,并成功测定了其ampD基因的序列;比对后发现阴沟肠杆菌、肺炎克雷伯菌、鲍曼不动杆菌和铜绿假单胞菌的染色体ampD基因的核苷酸序列差异较大.染色质ampD易突变位点分析统计表明,这些细菌的ampD基因的平均突变率分别为:64.5%、100.0%、97.0%和95.0%.结论 大部分临床常见产ampC β-内酰胺酶菌株含有ampD基因,并且有较高的突变率. |
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| AbstractList | Q814; 目的 了解临床常见产ampC β-内酰胺酶菌株中染色体ampD的分布及其突变位点.方法 41株临床常见产ampC β-内酰胺酶菌株(阴沟肠杆菌,肺炎克雷伯菌,鲍曼不动杆菌,铜绿假单胞菌)分离自医院感染样本,抽提其染色质DNA,PCR法扩增ampD基因并连接入pMD19-T载体后,再行PCR确定其存在.双链测序后比对染色体ampD基因的突变位点.结果 41株细菌的基因组中,使用PCR法扩增出染色体ampD基因有36株,并成功测定了其ampD基因的序列;比对后发现阴沟肠杆菌、肺炎克雷伯菌、鲍曼不动杆菌和铜绿假单胞菌的染色体ampD基因的核苷酸序列差异较大.染色质ampD易突变位点分析统计表明,这些细菌的ampD基因的平均突变率分别为:64.5%、100.0%、97.0%和95.0%.结论 大部分临床常见产ampC β-内酰胺酶菌株含有ampD基因,并且有较高的突变率. |
| Author | 方毅 庞立峰 王寅 赵虎 涂婉 |
| AuthorAffiliation | 复旦大学附属华东医院检验科,上海,200040%浙江中医药大学附属新华医院检验科,杭州,310005 |
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| Author_FL | TU Wan PANG Li-feng ZHAO Hu FANG Yi WANG Yin |
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| DocumentTitle_FL | Exploration of the mutations of genomic ampD from the clinical isolates producing ampC β-lactamases |
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| Snippet | Q814; 目的 了解临床常见产ampC β-内酰胺酶菌株中染色体ampD的分布及其突变位点.方法 41株临床常见产ampC... |
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| Title | 临床常见产ampC β-内酰胺酶菌株中染色质ampD基因突变位点的研究 |
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