miR-125b在鼻息肉上皮细胞中的表达及意义

R765.2; 目的 观察miR-125b在鼻息肉组织中的表达,并探讨其意义.方法 收集30例鼻息肉样本和16例正常下鼻甲样本,采用实时定量PCR法检测miR-125b表达;转染miR-125b模拟物(miR-125b mimics)至鼻息肉上皮细胞,分别采用CCK-8试验和流式细胞术检测miR-125b表达对鼻息肉上皮细胞增殖和凋亡的影响,Western blotting试验检测细胞中PI3K/Akt信号通路相关蛋白表达.结果 miR-125b在鼻息肉中表达低于正常下鼻甲(P＜0.05);在鼻息肉上皮细胞中过表达miR-125b则细胞增殖受到抑制、细胞凋亡增加(P均＜0.05),同时,细胞中...

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Published in山东医药 Vol. 58; no. 41; pp. 38 - 41
Main Authors 杨琳红, 王维峰, 张淑红, 范宗宪, 魏晓丽, 李江华
Format Magazine Article
LanguageChinese
Published 佳木斯大学附属第一医院,黑龙江佳木斯,154000%佳木斯大学基础医学院 2018
Subjects
细胞凋亡
鼻息肉
鼻息肉上皮细胞
微小RNA-125b
细胞增殖
Online AccessGet full text
ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2018.41.010

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Abstract R765.2; 目的 观察miR-125b在鼻息肉组织中的表达,并探讨其意义.方法 收集30例鼻息肉样本和16例正常下鼻甲样本,采用实时定量PCR法检测miR-125b表达;转染miR-125b模拟物(miR-125b mimics)至鼻息肉上皮细胞,分别采用CCK-8试验和流式细胞术检测miR-125b表达对鼻息肉上皮细胞增殖和凋亡的影响,Western blotting试验检测细胞中PI3K/Akt信号通路相关蛋白表达.结果 miR-125b在鼻息肉中表达低于正常下鼻甲(P＜0.05);在鼻息肉上皮细胞中过表达miR-125b则细胞增殖受到抑制、细胞凋亡增加(P均＜0.05),同时,细胞中PI3KCD和p-Akt蛋白表达均下降(P均＜0.05).结论 miR-125b在鼻息肉中表达降低,miR-125b可能通过负调控PI3K/Akt信号通路活性,促进鼻息肉上皮细胞增殖、抑制凋亡,进而诱导鼻息肉形成和发展.
AbstractList R765.2; 目的 观察miR-125b在鼻息肉组织中的表达,并探讨其意义.方法 收集30例鼻息肉样本和16例正常下鼻甲样本,采用实时定量PCR法检测miR-125b表达;转染miR-125b模拟物(miR-125b mimics)至鼻息肉上皮细胞,分别采用CCK-8试验和流式细胞术检测miR-125b表达对鼻息肉上皮细胞增殖和凋亡的影响,Western blotting试验检测细胞中PI3K/Akt信号通路相关蛋白表达.结果 miR-125b在鼻息肉中表达低于正常下鼻甲(P＜0.05);在鼻息肉上皮细胞中过表达miR-125b则细胞增殖受到抑制、细胞凋亡增加(P均＜0.05),同时,细胞中PI3KCD和p-Akt蛋白表达均下降(P均＜0.05).结论 miR-125b在鼻息肉中表达降低,miR-125b可能通过负调控PI3K/Akt信号通路活性,促进鼻息肉上皮细胞增殖、抑制凋亡,进而诱导鼻息肉形成和发展.
Author 范宗宪
张淑红
杨琳红
王维峰
魏晓丽
李江华
AuthorAffiliation 佳木斯大学附属第一医院,黑龙江佳木斯,154000%佳木斯大学基础医学院
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LI Jianghua
WEI Xiaoli
WANG Weifeng
YANG Linhong
ZHANG Shuhong
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Keywords 细胞凋亡
鼻息肉
鼻息肉上皮细胞
微小RNA-125b
细胞增殖
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Snippet R765.2; 目的 观察miR-125b在鼻息肉组织中的表达,并探讨其意义.方法 收集30例鼻息肉样本和16例正常下鼻甲样本,采用实时定量PCR法检测miR-125b表达;转染miR-125b模拟物(miR-125b...
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StartPage 38
Title miR-125b在鼻息肉上皮细胞中的表达及意义
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