99Tcm标记的小干扰RNA探针的制备及稳定性评价

R817.4; 通过偶联双功能螯合剂S-乙酰基-MAG3以达到制备99Tcm标记的小干扰RNA探针的目的,并对其标记条件及体外稳定性进行评价.结果表明,室温下99Tcm-siRNA在反应时间大于1 h、SnCl2·2H2O质量浓度小于2 g/L时,标记率可达(73.4±3.0)%;经Sephadex G25纯化后其放化纯度大于92%,比活度达25.9 PBq/mol;在室温和37 ℃条件下,99Tcm-siRNA于生理盐水和血清中均保持良好的标记稳定性;标记后的siRNA探针与未标记探针具备相似的靶向干扰活性....

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Published in核化学与放射化学 Vol. 32; no. 6; pp. 348 - 353
Main Authors 康磊, 王荣福, 闫平, 刘萌, 张春丽, 于明明, 崔永刚, 徐小洁
Format Journal Article
LanguageChinese
Published 北京大学第一医院,核医学科,北京,100034%军事医学科学院,生物工程研究所,北京,100850 2010
Subjects
99Tcm
小干扰RNA
标记
稳定性
Online AccessGet full text
ISSN0253-9950

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Abstract R817.4; 通过偶联双功能螯合剂S-乙酰基-MAG3以达到制备99Tcm标记的小干扰RNA探针的目的,并对其标记条件及体外稳定性进行评价.结果表明,室温下99Tcm-siRNA在反应时间大于1 h、SnCl2·2H2O质量浓度小于2 g/L时,标记率可达(73.4±3.0)%;经Sephadex G25纯化后其放化纯度大于92%,比活度达25.9 PBq/mol;在室温和37 ℃条件下,99Tcm-siRNA于生理盐水和血清中均保持良好的标记稳定性;标记后的siRNA探针与未标记探针具备相似的靶向干扰活性.
AbstractList R817.4; 通过偶联双功能螯合剂S-乙酰基-MAG3以达到制备99Tcm标记的小干扰RNA探针的目的,并对其标记条件及体外稳定性进行评价.结果表明,室温下99Tcm-siRNA在反应时间大于1 h、SnCl2·2H2O质量浓度小于2 g/L时,标记率可达(73.4±3.0)%;经Sephadex G25纯化后其放化纯度大于92%,比活度达25.9 PBq/mol;在室温和37 ℃条件下,99Tcm-siRNA于生理盐水和血清中均保持良好的标记稳定性;标记后的siRNA探针与未标记探针具备相似的靶向干扰活性.
Author 徐小洁
王荣福
康磊
张春丽
闫平
崔永刚
于明明
刘萌
AuthorAffiliation 北京大学第一医院,核医学科,北京,100034%军事医学科学院,生物工程研究所,北京,100850
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Author_FL KANG Lei
XU Xiao-jie
YU Ming-ming
LIU Meng
CUI Yong-gang
YAN Ping
WANG Rong-fu
ZHANG Chun-li
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小干扰RNA
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Snippet R817.4; 通过偶联双功能螯合剂S-乙酰基-MAG3以达到制备99Tcm标记的小干扰RNA探针的目的,并对其标记条件及体外稳定性进行评价.结果表明,室温下99Tcm-siRNA在反应时间大于1 h、SnCl2·2H2O质量浓度小于2 g/L时,标记率可达(73.4±3.0)%;经Sephadex...
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StartPage 348
Title 99Tcm标记的小干扰RNA探针的制备及稳定性评价
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