酸辅助分散液液微萃取-高效液相色谱-串联质谱法测定果汁中多种真菌毒素
建立了酸辅助分散液液微萃取结合高效液相色谱-串联质谱法检测果汁中黄曲霉毒素B1(Aflatoxin B1,AFB1)、黄曲霉毒素B2(Aflatoxin B2,AFB2)、黄曲霉毒素G1(Aflatoxin G1,AFG1)、黄曲霉毒素G2(Aflatoxin G2,AFG2)、赭曲霉毒素(Ochratoxin A,OTA)、桔霉素(Citrinin,CIT)、链格孢霉酚(Alternariol,AOH)和链格孢甲醚(Alternariol monomethyl Ether,AME)共8种真菌毒素的分析方法.考察了萃取剂的种类以及用量、分散剂用量、平衡体系pH值对萃取效率的影响,获得了最佳的分...
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| Published in | 分析化学 Vol. 47; no. 3; pp. 455 - 462 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
天然农药与化学生物学教育部重点实验室,广东省生物农药创制与应用重点实验室,华南农业大学农学院,广州510642%广东检验检疫技术中心,广州,510623
2019
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.19756/j.issn.0253-3820.181299 |
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| Abstract | 建立了酸辅助分散液液微萃取结合高效液相色谱-串联质谱法检测果汁中黄曲霉毒素B1(Aflatoxin B1,AFB1)、黄曲霉毒素B2(Aflatoxin B2,AFB2)、黄曲霉毒素G1(Aflatoxin G1,AFG1)、黄曲霉毒素G2(Aflatoxin G2,AFG2)、赭曲霉毒素(Ochratoxin A,OTA)、桔霉素(Citrinin,CIT)、链格孢霉酚(Alternariol,AOH)和链格孢甲醚(Alternariol monomethyl Ether,AME)共8种真菌毒素的分析方法.考察了萃取剂的种类以及用量、分散剂用量、平衡体系pH值对萃取效率的影响,获得了最佳的分散液液微萃取(Dispersive liquid-liquid microextraction,DLLME)条件:萃取剂为三氯苯胺,用量为60μL;分散剂为HCl溶液,用量为1500μL;平衡体系pH=5.结果表明,8种真菌毒素在1～500μg/L范围内呈良好的线性关系,相关系数>0.994;在芒果、菠萝和火龙果果汁中的检出限分别为0.15～17.71 ng/L、0.09～7.08 ng/L和0.40～14.29 ng/L;当添加浓度为0.2、1.0和10.0μg/L时,回收率在75.6％ ～110.0％之间,相对标准偏差<15.5％.本方法简单、快速,有机溶剂用量少,灵敏度高,重现性好,可用于果汁中多种痕量真菌毒素的检测. |
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| AbstractList | 建立了酸辅助分散液液微萃取结合高效液相色谱-串联质谱法检测果汁中黄曲霉毒素B1(Aflatoxin B1,AFB1)、黄曲霉毒素B2(Aflatoxin B2,AFB2)、黄曲霉毒素G1(Aflatoxin G1,AFG1)、黄曲霉毒素G2(Aflatoxin G2,AFG2)、赭曲霉毒素(Ochratoxin A,OTA)、桔霉素(Citrinin,CIT)、链格孢霉酚(Alternariol,AOH)和链格孢甲醚(Alternariol monomethyl Ether,AME)共8种真菌毒素的分析方法.考察了萃取剂的种类以及用量、分散剂用量、平衡体系pH值对萃取效率的影响,获得了最佳的分散液液微萃取(Dispersive liquid-liquid microextraction,DLLME)条件:萃取剂为三氯苯胺,用量为60μL;分散剂为HCl溶液,用量为1500μL;平衡体系pH=5.结果表明,8种真菌毒素在1～500μg/L范围内呈良好的线性关系,相关系数>0.994;在芒果、菠萝和火龙果果汁中的检出限分别为0.15～17.71 ng/L、0.09～7.08 ng/L和0.40～14.29 ng/L;当添加浓度为0.2、1.0和10.0μg/L时,回收率在75.6％ ～110.0％之间,相对标准偏差<15.5％.本方法简单、快速,有机溶剂用量少,灵敏度高,重现性好,可用于果汁中多种痕量真菌毒素的检测. |
| Author | 邓年 刘承兰 谢建军 韩艺烨 |
| AuthorAffiliation | 天然农药与化学生物学教育部重点实验室,广东省生物农药创制与应用重点实验室,华南农业大学农学院,广州510642%广东检验检疫技术中心,广州,510623 |
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| Author_FL | LIU Cheng-Lan DENG Nian HAN Yi-Ye XIE Jian-Jun |
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| DocumentTitle_FL | Acid-assisted Dispersive Liquid-Liquid Microextraction- High Performance Liquid Chromatography-Tandem Mass Spectrometry for Determination of Mycotoxins in Fruit Juice |
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| Keywords | 酸辅助-分散液液微萃取 真菌毒素 果汁 高效液相色谱-串联质谱法 |
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| Title | 酸辅助分散液液微萃取-高效液相色谱-串联质谱法测定果汁中多种真菌毒素 |
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