以碳量子点为过氧化物模拟酶的葡萄糖测定方法
以烟炱为原料制备水溶性碳量子点(CQDs),此碳量子点具有过氧化物模拟酶活性,可催化H2O2氧化3,3′,5,5′-四甲基联苯胺(TMB)产生显色反应,而葡萄糖氧化酶(GOx)可催化葡萄糖氧化产生H2O2,基于上述原理,建立了一种以CQDs为过氧化物模拟酶的葡萄糖测定方法.以TMB为底物,研究了温度、 pH值等条件对催化性能的影响.结果表明,pH 3. 5、 35℃、 0. 5 mmol/L TMB、 1 μg/mL CQDs时,此方法检测葡萄糖的线性范围为0. 025~0. 40 mmol/L,检出限为5. 10 μmol/L(S/N=3),尿液中的常见干扰物不干扰测定.实际尿液中的加标回收...
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| Published in | 分析化学 Vol. 46; no. 7; pp. 1062 - 1068 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西大学化学化工学院,广西生物炼制重点实验室,南宁530004
2018
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.11895/j.issn.0253-3820.171396 |
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| Abstract | 以烟炱为原料制备水溶性碳量子点(CQDs),此碳量子点具有过氧化物模拟酶活性,可催化H2O2氧化3,3′,5,5′-四甲基联苯胺(TMB)产生显色反应,而葡萄糖氧化酶(GOx)可催化葡萄糖氧化产生H2O2,基于上述原理,建立了一种以CQDs为过氧化物模拟酶的葡萄糖测定方法.以TMB为底物,研究了温度、 pH值等条件对催化性能的影响.结果表明,pH 3. 5、 35℃、 0. 5 mmol/L TMB、 1 μg/mL CQDs时,此方法检测葡萄糖的线性范围为0. 025~0. 40 mmol/L,检出限为5. 10 μmol/L(S/N=3),尿液中的常见干扰物不干扰测定.实际尿液中的加标回收率为95. 0% ~105. 1% . |
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| AbstractList | 以烟炱为原料制备水溶性碳量子点(CQDs),此碳量子点具有过氧化物模拟酶活性,可催化H2O2氧化3,3′,5,5′-四甲基联苯胺(TMB)产生显色反应,而葡萄糖氧化酶(GOx)可催化葡萄糖氧化产生H2O2,基于上述原理,建立了一种以CQDs为过氧化物模拟酶的葡萄糖测定方法.以TMB为底物,研究了温度、 pH值等条件对催化性能的影响.结果表明,pH 3. 5、 35℃、 0. 5 mmol/L TMB、 1 μg/mL CQDs时,此方法检测葡萄糖的线性范围为0. 025~0. 40 mmol/L,检出限为5. 10 μmol/L(S/N=3),尿液中的常见干扰物不干扰测定.实际尿液中的加标回收率为95. 0% ~105. 1% . |
| Abstract_FL | Water soluble carbon quantum dots ( CQDs) were prepared by using soot as carbon source. The obtained CQDs showed an excellent intrinsic peroxidase-like activity, which could catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2and thus resulted in color change. Glucose could react with dissolved oxygen to produce H2O2in the presence of glucose oxidase ( GOx) . A colorimetric method using CQDs as peroxidase mimetic enzyme was developed for glucose determination. When TMB was acted as a substrate, the effect of a series of conditions, such as temperature and pH on the catalytic activity of the obtained CQDs, was systematically studied. Under optimal conditions, e. g. pH 3. 5 and temperature 35℃, 0. 5 mmol/L TMB and 1 μg/mL CQDs, the absorbance at 652 nm showed linear response with glucose concentrations ranging from 0. 025 mmol/L to 0. 40 mmol/L, with detection limit of 5. 10 μmol/L (3σ/k). The proposed method exhibited excellent selectivity and the common substances did not interfere with detection of glucose. This method was successfully applied to detect glucose in real samples with recoveries of 95. 0%-105. 1% . |
| Author | 刘馨宇 钟青梅 陈羽烨 王益林 黄欣虹 覃庆敏 苏安梅 |
| AuthorAffiliation | 广西大学化学化工学院,广西生物炼制重点实验室,南宁530004 |
| AuthorAffiliation_xml | – name: 广西大学化学化工学院,广西生物炼制重点实验室,南宁530004 |
| Author_FL | SU An-Mei LIU Xin-Yu ZHONG Qing-Mei HUANG Xin-Hong CHEN Yu-Ye QIN Qing-Min WANG Yi-Lin |
| Author_FL_xml | – sequence: 1 fullname: ZHONG Qing-Mei – sequence: 2 fullname: HUANG Xin-Hong – sequence: 3 fullname: QIN Qing-Min – sequence: 4 fullname: SU An-Mei – sequence: 5 fullname: CHEN Yu-Ye – sequence: 6 fullname: LIU Xin-Yu – sequence: 7 fullname: WANG Yi-Lin |
| Author_xml | – sequence: 1 fullname: 钟青梅 – sequence: 2 fullname: 黄欣虹 – sequence: 3 fullname: 覃庆敏 – sequence: 4 fullname: 苏安梅 – sequence: 5 fullname: 陈羽烨 – sequence: 6 fullname: 刘馨宇 – sequence: 7 fullname: 王益林 |
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| DOI | 10.11895/j.issn.0253-3820.171396 |
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| DocumentTitle_FL | Determination of Glucose Based on Carbon Quantum Dots as Peroxidase Mimetic Enzyme |
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| GrantInformation_xml | – fundername: 广西省自然科学基金项目; 广西生物炼制重点实验室开放基金; 广西大学大学生创新创业训练计划项目(No. 201710593180)资助 This work was supported by the Natural Science Foundation of Guangxi Province, China; the Opening Project of Guangxi Key Laboratory of Biorefinery; the Foundation of College Student Experimental Skills and Innovation Ability Training of Guangxi University, China funderid: (2014GXNSFAA118328); (GXKLB-2); (2014GXNSFAA118328); (GXKLB-2); (201710593180) |
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| Keywords | Mimetic enzyme 模拟酶 葡萄糖 碳量子点 Carbon quantum dots Glucose |
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| Snippet | 以烟炱为原料制备水溶性碳量子点(CQDs),此碳量子点具有过氧化物模拟酶活性,可催化H2O2氧化3,3′,5,5′-四甲基联苯胺(TMB)产生显色反应,而葡萄糖氧化酶(GOx)可催化葡萄糖氧化产生H2O2,基于上述原理,建立了一种以CQDs为过氧化物模拟酶的葡萄糖测定方法.以TMB为底物,研究了温度、... |
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| Title | 以碳量子点为过氧化物模拟酶的葡萄糖测定方法 |
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