薯蓣皂苷元通过miR-145去甲基化抑制MCF-7乳腺癌细胞增殖与迁移

R285.5; 目的 探讨薯蓣皂苷元(Diosgenin)对MCF-7乳腺癌细胞的作用及可能机制.方法 选取人正常乳腺上皮细胞系MCF-10 A和人乳腺癌细胞系MCF-7进行实验,加入不同浓度的薯蓣皂苷元,MTS法检测薯蓣皂苷元对细胞增殖的影响,划痕实验测定薯蓣皂苷元对乳腺癌细胞迁移能力的影响,甲基化特异性PCR(MSP)方法明确薯蓣皂苷元对MCF-7细胞微小RNA-145(miR-145)甲基化的作用,实时荧光定量PCR(qPCR)检测MCF-7细胞miR-145 mRNA表达情况.结果 薯蓣皂苷元可显著抑制乳腺癌细胞系MCF-7的增殖,且呈浓度和时间依赖性,其增殖抑制率明显高于正常乳腺上皮...

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Published in北京中医药大学学报 Vol. 42; no. 8; pp. 662 - 666
Main Authors 李亚, 吕鹏, 侯丽, 张雅月, 王冲, 范秋月, 陈信义, 石凤芹
Format Journal Article
LanguageChinese
Published 北京中医药大学东直门医院 北京100700 2019
Subjects
微小RNA-145
去甲基化
乳腺癌
薯蓣皂苷元
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Abstract R285.5; 目的 探讨薯蓣皂苷元(Diosgenin)对MCF-7乳腺癌细胞的作用及可能机制.方法 选取人正常乳腺上皮细胞系MCF-10 A和人乳腺癌细胞系MCF-7进行实验,加入不同浓度的薯蓣皂苷元,MTS法检测薯蓣皂苷元对细胞增殖的影响,划痕实验测定薯蓣皂苷元对乳腺癌细胞迁移能力的影响,甲基化特异性PCR(MSP)方法明确薯蓣皂苷元对MCF-7细胞微小RNA-145(miR-145)甲基化的作用,实时荧光定量PCR(qPCR)检测MCF-7细胞miR-145 mRNA表达情况.结果 薯蓣皂苷元可显著抑制乳腺癌细胞系MCF-7的增殖,且呈浓度和时间依赖性,其增殖抑制率明显高于正常乳腺上皮细胞MCF-10A(P<0.05).薯蓣皂苷元能明显减弱MCF-7细胞迁移能力,呈浓度依赖性(P<0.01).与对照组相比,经薯蓣皂苷元作用后MCF-7细胞中miR-145表现为去甲基化状态,且miR-145 mRNA表达显著升高(P<0.01),其升高程度与浓度正相关.结论 薯蓣皂苷元能抑制乳腺癌增殖与迁移,其机制可能与调控miR-145表达水平及甲基化状态相关.基于对miR-145的去甲基化从而发挥抑制肿瘤作用.
AbstractList R285.5; 目的 探讨薯蓣皂苷元(Diosgenin)对MCF-7乳腺癌细胞的作用及可能机制.方法 选取人正常乳腺上皮细胞系MCF-10 A和人乳腺癌细胞系MCF-7进行实验,加入不同浓度的薯蓣皂苷元,MTS法检测薯蓣皂苷元对细胞增殖的影响,划痕实验测定薯蓣皂苷元对乳腺癌细胞迁移能力的影响,甲基化特异性PCR(MSP)方法明确薯蓣皂苷元对MCF-7细胞微小RNA-145(miR-145)甲基化的作用,实时荧光定量PCR(qPCR)检测MCF-7细胞miR-145 mRNA表达情况.结果 薯蓣皂苷元可显著抑制乳腺癌细胞系MCF-7的增殖,且呈浓度和时间依赖性,其增殖抑制率明显高于正常乳腺上皮细胞MCF-10A(P<0.05).薯蓣皂苷元能明显减弱MCF-7细胞迁移能力,呈浓度依赖性(P<0.01).与对照组相比,经薯蓣皂苷元作用后MCF-7细胞中miR-145表现为去甲基化状态,且miR-145 mRNA表达显著升高(P<0.01),其升高程度与浓度正相关.结论 薯蓣皂苷元能抑制乳腺癌增殖与迁移,其机制可能与调控miR-145表达水平及甲基化状态相关.基于对miR-145的去甲基化从而发挥抑制肿瘤作用.
Author 李亚
吕鹏
张雅月
石凤芹
侯丽
王冲
范秋月
陈信义
AuthorAffiliation 北京中医药大学东直门医院 北京100700
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Author_FL Zhang Yayue
Hou Li
Li Ya
Wang Chong
Chen Xinyi
Fan Qiuyue
Lyu Peng
Shi Fengqin
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  fullname: 陈信义
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  fullname: 石凤芹
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DocumentTitle_FL Diosgenin' s inhibitory effects on proliferation and migration of MCF-7 breast cancer cells through demethylation of microRNA-145
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Keywords 微小RNA-145
去甲基化
乳腺癌
薯蓣皂苷元
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Snippet R285.5; 目的 探讨薯蓣皂苷元(Diosgenin)对MCF-7乳腺癌细胞的作用及可能机制.方法 选取人正常乳腺上皮细胞系MCF-10...
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Title 薯蓣皂苷元通过miR-145去甲基化抑制MCF-7乳腺癌细胞增殖与迁移
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