Luteinizing hormone inhibits Fas-induced apoptosis in ovarian surface epithelial cell lines
Gonadotrophins including LH have been suggested to play an important role in the etiology of epithelial ovarian cancers. The goal of the present study was to obtain more insight in the mechanism of gonadotrophin action on ovarian surface epithelium (OSE) cells. As the Fas system is known to be a maj...
Saved in:
Published in | Journal of endocrinology Vol. 188; no. 3 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Published |
2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Gonadotrophins including LH have been suggested to play an important role in the etiology of epithelial ovarian cancers. The goal of the present study was to obtain more insight in the mechanism of gonadotrophin action on ovarian surface epithelium (OSE) cells. As the Fas system is known to be a major player in the regulation of the process of apoptosis in the ovary, we investigated whether LH interfered with Fas-induced apoptosis in the human OSE cancer cell lines HEY and Caov-3. Activation of Fas receptor by an agonistic anti-Fas receptor antibody induced apoptosis, as was evaluated by caspase-3 activation, poly(ADP-ribose) polymerase fragmentation, phosphatidylserine externalization and morphological changes characteristic of apoptosis. Co-treatment with LH reduced the number of apoptotic cells following activation of Fas in a transient manner, while LH by itself did not affect apoptosis or cell proliferation. The anti-apoptotic effect of LH could be mimicked by the membrane-permeable cAMP analog 8-(4-chlorophenylthio) cAMP (8-CPT-cAMP), and blocked by H89, a specific inhibitor of protein kinase A (PKA). In conclusion, these findings suggest that LH protects HEY cells against Fas-induced apoptosis through a signaling cascade involving PKA. Although it is plausible that in vivo LH might also enhance OSE tumor growth through inhibition of apoptosis, further research is necessary to confirm this hypothesis. |
---|---|
AbstractList | Gonadotrophins including LH have been suggested to play an important role in the etiology of epithelial ovarian cancers. The goal of the present study was to obtain more insight in the mechanism of gonadotrophin action on ovarian surface epithelium (OSE) cells. As the Fas system is known to be a major player in the regulation of the process of apoptosis in the ovary, we investigated whether LH interfered with Fas-induced apoptosis in the human OSE cancer cell lines HEY and Caov-3. Activation of Fas receptor by an agonistic anti-Fas receptor antibody induced apoptosis, as was evaluated by caspase-3 activation, poly(ADP-ribose) polymerase fragmentation, phosphatidylserine externalization and morphological changes characteristic of apoptosis. Co-treatment with LH reduced the number of apoptotic cells following activation of Fas in a transient manner, while LH by itself did not affect apoptosis or cell proliferation. The anti-apoptotic effect of LH could be mimicked by the membrane-permeable cAMP analog 8-(4-chlorophenylthio) cAMP (8-CPT-cAMP), and blocked by H89, a specific inhibitor of protein kinase A (PKA). In conclusion, these findings suggest that LH protects HEY cells against Fas-induced apoptosis through a signaling cascade involving PKA. Although it is plausible that in vivo LH might also enhance OSE tumor growth through inhibition of apoptosis, further research is necessary to confirm this hypothesis. |
Author | Boer-Brouwer, M., de Teerds, K.J Houweling, M Slot, K.A Vaandrager, A.B Dorrington, J.H |
Author_xml | – sequence: 1 fullname: Slot, K.A – sequence: 2 fullname: Boer-Brouwer, M., de – sequence: 3 fullname: Houweling, M – sequence: 4 fullname: Vaandrager, A.B – sequence: 5 fullname: Dorrington, J.H – sequence: 6 fullname: Teerds, K.J |
BookMark | eNqljcFqwkAQQJeiYNT-w_xAYGOMabyWSg899tbDMomjGVlnw0620n59U_APPL3D4_GWZiZB6MlkxbZu8t2LrWYms3azyW3dVAuzVL1YW1RFXWbm6yONxMK_LGfoQ7xOKbD03PKocEDNWY6poyPgEIYxKOukIXxjZBTQFE_YEdDAY0-e0UNH3oNnIV2b-Qm90vOdK7M_vH2-vuc3PJNMQxInGDtWF5Cd5zZi_HG3FJ34fwypVVduq7Kx5UPxHxH1W4k |
ContentType | Journal Article |
Copyright | Wageningen University & Research |
Copyright_xml | – notice: Wageningen University & Research |
DBID | QVL |
DatabaseName | NARCIS:Publications |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Anatomy & Physiology |
EISSN | 1479-6805 |
ExternalDocumentID | oai_library_wur_nl_wurpubs_345390 |
GroupedDBID | - 02 08R 0R 2WC 3O- 4.4 53G 55 5GY 5RE 5VS AAFZV AAYJJ ABFLS ABLYK ABOCM ABPTK ABSGY ACNCT ACPRK ADACO ADBBV ADBIT ADDZX AENEX AFDAS AFFNX AGCAB ALMA_UNASSIGNED_HOLDINGS BAWUL CS3 DIK DU5 E3Z EBS EJD F5P FA8 FH7 GJ GX1 H13 HZ H~9 IL9 INIJC J5H K-O KM KQ8 L7B MVM O0- O9- OHT OK1 P2P QVL REN RHF TBS VH1 WH7 WOQ X X7M XJT ZA5 ZGI ZXP |
ID | FETCH-wageningen_narcis_oai_library_wur_nl_wurpubs_3453903 |
ISSN | 0022-0795 |
IngestDate | Tue Jan 05 18:16:41 EST 2021 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-wageningen_narcis_oai_library_wur_nl_wurpubs_3453903 |
ParticipantIDs | wageningen_narcis_oai_library_wur_nl_wurpubs_345390 |
ProviderPackageCode | QVL |
PublicationCentury | 2000 |
PublicationDate | 2006 |
PublicationDateYYYYMMDD | 2006-01-01 |
PublicationDate_xml | – year: 2006 text: 2006 |
PublicationDecade | 2000 |
PublicationTitle | Journal of endocrinology |
PublicationYear | 2006 |
SSID | ssj0015173 |
Score | 3.6176107 |
Snippet | Gonadotrophins including LH have been suggested to play an important role in the etiology of epithelial ovarian cancers. The goal of the present study was to... |
SourceID | wageningen |
SourceType | Open Access Repository |
SubjectTerms | cancer-cells cyclic-amp follicle-stimulating-hormone gene-expression gonadotropin receptor granulosa-cells growth-factor-i incessant ovulation neutrophil apoptosis protein-kinase-a |
Title | Luteinizing hormone inhibits Fas-induced apoptosis in ovarian surface epithelial cell lines |
URI | http://www.narcis.nl/publication/RecordID/oai:library.wur.nl:wurpubs%2F345390 |
Volume | 188 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT8JAEN4QDwYPRkHjO3swXkwJsC223tBIiIgX0ZB4aLbtEkiwJX1I5Nc7syx9GI3KpW3atCz77c7OfDsPQs5hzRCmsJjGvJYFBooOcw4WBs0ZucKsO45lWBiN3H9sdZ_1-6ExLJV6Oa-lJHZq7uLbuJJ1UIV7gCtGyf4D2fSjcAOuAV84AsJw_BPGDwnWqpws0Nwfg_IZyBQg44mDuwEdHmlgcCe4wc9nwSwOMPfIBNTDd7CPYVpHSTjiMK3FDOMypkidI41_iYpn9IPSKnwvADlTJOOfpoEU5b1arlqxCDUw8dEHX3KuUgh52SjCJ1NVTyUlf144970Qs2pIkVW7KXISeQnbRG_WZeHMmlgKVf3K0lpm3ShKXTM3vFi2HK224L-sUoV82IrhsudJaPtTPMHgjWymG8xC_gZTBmHg9zB19wHFRnkbqPZtkfIc_o8vQ9hyKsVgh2yrbqXtJbC7pCT8Cqm2fR4Hbx_0gkrvXNnTFbLZV04QVfKag50q2OkKdpqDnaaww2OqYKcKdprBThF2KmHfI9edu8FtV8tabftYbiqyf-0Rtk82fGjLAaFoIDS4bgpDMJ0bdcdtMKfpeoYr6tw0W4eErfEDR2u9dUzKGaF1QjbiMBGnoOLFzpnE7hOx02kn |
link.rule.ids | 230,315,786,790,891,4043 |
linkProvider | Geneva Foundation for Medical Education and Research |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Luteinizing+hormone+inhibits+Fas-induced+apoptosis+in+ovarian+surface+epithelial+cell+lines&rft.jtitle=Journal+of+endocrinology&rft.au=Slot%2C+K.A&rft.au=Boer-Brouwer%2C+M.%2C+de&rft.au=Houweling%2C+M&rft.au=Vaandrager%2C+A.B&rft.date=2006&rft.issn=0022-0795&rft.eissn=1479-6805&rft.volume=188&rft.issue=3&rft.externalDBID=n%2Fa&rft.externalDocID=oai_library_wur_nl_wurpubs_345390 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-0795&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-0795&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-0795&client=summon |