Compositions and methods for diagnosing tumors using LEDGF/p75
The invention provides methods for diagnosing tumors in mammals using a reagent that bind to LEDGF/p75 or to a nucleic acid encoding LEDGF/p75. For example, the tumor may be located in the CNS, the prostate, the skin, the bone marrow, or the gut of the mammal. Also provided are methods for diagnosin...
Saved in:
| Main Authors | , |
|---|---|
| Format | Patent |
| Language | English |
| Published |
01.05.2012
|
| Online Access | Get full text |
Cover
Loading…
| Abstract | The invention provides methods for diagnosing tumors in mammals using a reagent that bind to LEDGF/p75 or to a nucleic acid encoding LEDGF/p75. For example, the tumor may be located in the CNS, the prostate, the skin, the bone marrow, or the gut of the mammal. Also provided are methods for diagnosing brain tumors such as medulloblastomas, meningiomas, astrocytomas, glioblastomas multiforme, and ependymomas by examining LEDGF/p75 or a nucleic acid encoding LEDGF/p75 localization. The invention also involves methods for diagnosing cancers involving cancerous epithelial cells such as colon cancer. The instant invention also provides methods for isolating stem cells from a heterogeneous population of cells, as well as methods for identifying neuroepithelial stem cells, newly differentiated neurons, and astrocytes in a subject. Also provided are methods for inducing the differentiation of neuroepithelial stem cells into astrocytes or neurons and methods for screening candidate compounds that regulate the differentiation of neuroepithelial stem cells. |
|---|---|
| AbstractList | The invention provides methods for diagnosing tumors in mammals using a reagent that bind to LEDGF/p75 or to a nucleic acid encoding LEDGF/p75. For example, the tumor may be located in the CNS, the prostate, the skin, the bone marrow, or the gut of the mammal. Also provided are methods for diagnosing brain tumors such as medulloblastomas, meningiomas, astrocytomas, glioblastomas multiforme, and ependymomas by examining LEDGF/p75 or a nucleic acid encoding LEDGF/p75 localization. The invention also involves methods for diagnosing cancers involving cancerous epithelial cells such as colon cancer. The instant invention also provides methods for isolating stem cells from a heterogeneous population of cells, as well as methods for identifying neuroepithelial stem cells, newly differentiated neurons, and astrocytes in a subject. Also provided are methods for inducing the differentiation of neuroepithelial stem cells into astrocytes or neurons and methods for screening candidate compounds that regulate the differentiation of neuroepithelial stem cells. |
| Author | Goldstein, Lee E Fu, Ling |
| Author_xml | – sequence: 1 givenname: Lee E surname: Goldstein fullname: Goldstein, Lee E – sequence: 2 givenname: Ling surname: Fu fullname: Fu, Ling |
| BookMark | eNrjYmDJy89L5WSwc87PLcgvzizJzM8rVkjMS1HITS3JyE8pVkjLL1JIyUxMzwPK5qUrlJTm5hcVK5SCOT6uLu5u-gXmpjwMrGmJOcWpvFCam0HBzTXE2UO3tLggsSQ1r6Q4Pr0oEUQZWBiaWRhbGhsToQQAA50ykg |
| ContentType | Patent |
| CorporateAuthor | The Brigham and Women's Hospital, Inc |
| CorporateAuthor_xml | – name: The Brigham and Women's Hospital, Inc |
| DBID | EFH |
| DatabaseName | USPTO Issued Patents |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: EFH name: USPTO Issued Patents url: http://www.uspto.gov/patft/index.html sourceTypes: Open Access Repository |
| DeliveryMethod | fulltext_linktorsrc |
| ExternalDocumentID | 08168393 |
| GroupedDBID | EFH |
| ID | FETCH-uspatents_grants_081683933 |
| IEDL.DBID | EFH |
| IngestDate | Sun Mar 05 22:33:05 EST 2023 |
| IsOpenAccess | true |
| IsPeerReviewed | false |
| IsScholarly | false |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-uspatents_grants_081683933 |
| OpenAccessLink | https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8168393 |
| ParticipantIDs | uspatents_grants_08168393 |
| PatentNumber | 8168393 |
| PublicationCentury | 2000 |
| PublicationDate | 20120501 |
| PublicationDateYYYYMMDD | 2012-05-01 |
| PublicationDate_xml | – month: 05 year: 2012 text: 20120501 day: 01 |
| PublicationDecade | 2010 |
| PublicationYear | 2012 |
| References | Pena et al. (2004/0038348) 20040200 (WO 2005/005601) 20050100 Monti et al., "NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development", Eur. J. Neurosci., 16:1490-1498 (2002). Crino et al., "Presence and phosphorylation of transcription factors in developing dendrites", Proc. Natl. Acad. Sci. USA, 95:2313-2318 (1998). (0 438 520) 19910700 Goldstein et al., "Beta-Amyloid Promotes Lens Protein Aggregation and is Associated with Supranuclear Lens Opacification", Invest. Ophthalmol. Vis. Sci., 43 (2002) (Abstract Only). Fu et al., "Differential Expression of Lens Epithelial-derived Growth Factor (LEDGF) During Neuroepithelial Stem Cell Differentiation in The CNS", ARVO Meeting Abstracts, 45:1719 (May 1, 2004) (Abstract Only). Llano et al., "LEDGF/p75 Determines Cellular Trafficking of Diverse Lentiviral but Not Murine Oncoretroviral Integrase Proteins and Is a Component of Functional Lentiviral Preintegration Complexes", J. Virol., 78(17):9524-9537 (2004). Singh et al., "Lens Epithemlium-Derived Growth Factor: Increased Resistance to Thermal and Oxidative Stresses", Invest. Ophthalmol. Vis. Sci., 40(7):1444-1451 (1999). (WO 2005/015236) 20050200 GenBank Accession No. AF098482, Dec. 23, 1998. Daniels et al., "Antinuclear Autoantibodies in Prostate Cancer: Immunity to LEDGF/p75, a Survival Protein Highly Expressed in Prostate Tumors and Cleaved During Apoptosis", Prostate, 62:14-26 (2005). Kalpana (2004/0091487) 20040500 Johe et al., "Single factors direct the differentiation of stem cells from the fetal and adult central nervous system", Genes Dev., 10:3129-3140 (1996). Murphy et al., "Morphological plasticity of dendritic spines in central neurons is mediated by activation of cAMP response element binding protein", Proc. Natl. Acad. Sci. USA, 94:1482-1487 (1997). Riccio et al., "Mediation by a CREB Family Transcription Factor of NGF-Dependent Survival of Sympathetic Neurons", Science, 286:2358-2361 (1999). Lepourcelet et al., "Insights into developmental mechanisms and cancers in the mammalian intestine derived from serial analysis of gene expression and study of the hepatoma-derived growth factor (HDGF)", Develop. Dis., 32(2):415-427 (2005). Busschots et al., "The Interaction of LEDGF/p75 with Integrase is Lentivirus-specific and Promotes DNA Binding", J. Biol. Chem., 280(18):17841-17847 (2005). Goldstein et al., "Alzheimer's Disease β-Amyloid (Aβ) in the Lens: Interactions with AlphaB-Crystallin, Small Heat Schock Proteins, and Redox-Active Metal", Invest. Ophthalmol. Vis. Sci., 45:3507 (2004) (Abstract Only). Lu et al. (2004/0142333) 20040700 Kubo et al., "Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells", Histochem. Cell Biol., 119:289-299 (2003). Chylack et al., "Lens epithelium-derived growth factor (LEDGF/p75) expression in fetal and adult human brain", Exp. Eye Res., 79:941-948 (2004). GenBank Accession No. AAC25167, Mar. 23, 2001. Walton et al., "Immediate Early Gene Transcription and Synaptic Modulation", J. Neurosci. Res., 58(1):96-106 (1999). Lonze et al., "Function and Regulation of CREB Family Transcription Factors in the Nervous System", Neuron, 35:605-623 (2002). Daugaard et al. (2007). Lens epithelium-derived growth factor is an Hsp70-2 regulated guardian of lysosomal stability in human cancer. Cancer. Res. 67(6):2559-2567. Greenwood et al. (2003/0059868) 20030300 Dietz et al., "The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies", Biochem. J., 366:491-500 (2002). Nakagawa et al., "Regulation of Neurogenesis in Adult Mouse Hippocampus by cAMP and the cAMP Response Element-Binding Protein", J. Neurosci.,22(9):3673-3682 (2002). Ge et al., "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation", Embo J., 17(22):6723-6729 (1998). Pena et al. (2004/0087505) 20040500 Kirschenbaum et al., "In vitro Neuronal Production and Differentiation by Precursor Cells Derived from the Adult Human Forebrain", Cereb. Cortex, 4(6):576-589 (1994). Llano et al., "Lens Epithelium-derived Growth Factor/p75 Prevents Proteasomal Degradation of HIV-1 Integrase", J. Biol. Chem., 279(53):55570-55577 (2004). Fujioka et al., "Activation of cAMP Signaling Facilitates the Morphological Maturation of Newborn Neurons in Adult Hippocampus", J. Neurosci., 24(2):319-328 (2004). Singh et al., "Lens Epithelium-Derived Growth Factor: Effects on Growth and Survival of Lens Epithelial Cells, Keratinocytes, and Fibroblasts", Biochem. Biophys. Res. Commun., 267:373-381 (2000). Hussey et al., "Fusion of the NUP98 gene with the LEDGF/p52 gene defines a recurrent acute myeloid leukemia translocation", BMC Genetics, 2:20-23 (2001). GenBank GeneID:11168, updated Feb. 3, 2008. Goldstein et al., "Lens Epithelium-Derived Growth Factor (LEDGF) in the Retina and Brain", Invest. Ophthalmol. Vis. Sci., 41(4):S870, Abstract No. 4626-B573 (2000). (WO 90/07380) 19900700 Mancini et al., "Lens Epithelial-Derived Growth Factor (LEDGF) is Expressed in Neurons Within the Mammalian Central Nervous System", Invest. Ophthalmol. Vis. Sci., 44:E-Abstract 4509 (2003) (Abstract Only). Sikes et al. (2002/0155119) 20021000 Buc-Caron, M.H., "Neuroepithelial progenitor cells explanted from human fetal brain proliferate and differentiate in vitro", Neurobiol. Dis., 2:37-47 (1995). Abe et al. (2003/0008330) 20030100 (WO 96/26782) 19960900 Fatma et al., "Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, by Lens Epithelium-derived Growth Factor to Protect Cells from Oxidative Stress", J. Biol. Chem., 276(52):48899-48907 (2001). Pons et al., "Vitronectin regulates Sonic hedgehog activity during cerebellum development through CREB phosphorylation", Development, 128:1481-1492 (2001). Vanegas et al., "Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering", J. Cell Sci., 118(Pt. 8):1733-1743 (2005). Burgess et al. (2003/0190708) 20031000 Walton et al., "CREB Phosphorylation Promotes Nerve Cell Survival", J. Neurochem., 73(5):1836-1842 (1999). Maertens et al., "Identification and Characterization of a Functional Nuclear Localization Signal in the HIV-1 Integrase Interactor LEDGF/p75", J. Biol. Chem., 279(32):33421-33429 (2004). Rubinson et al., "A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference", Nat. Genet., 33(3):401-406 (2003). Bernier et al., "Characterization of the subventricular zone of the adult human brain: evidence for the involvement of Bcl-2" , Neurosci. Res., 37(1):67-78 (2000). Tan et al. (2003/0215875) 20031100 (WO 00/29578) 20000500 Maertens et al., "Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75", FASEB J., 19(8):1039-1041 (2005). GenBank Accession No. AAF25871, Jul. 31, 2002. Shinohara et al. (6750052) 20040600 Emiliani et al., "Integrase Mutants Defective for Interaction with LEDGF/p75 Are Impaired in Chromosome Tethering and HIV-1 Replication", J. Biol. Chem., 280(27):25517-25523 (2005). GenBank Accession No. AAF25870, Jul. 31, 2002. Giachino et al., "cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb", J. Neurosci., 25(44):10105-10118 (2005). Eriksson et al., "Neurogenesis in the adult human hippocampus", Nat. Med., 4(11):1313-1317 (1998). Burgess et al. (6699967) 20040300 Singh et al., "LEDGF Binds to Heat Shock and Stress-Related Element to Activate the Expression of Stress-Related Genes", Biochem. Biophys. Res. Commun., 283:943-955 (2001). Mancini et al., "Lens epithelial-derived growth factor (LEDGF) is differentially expressed and localized in mammalian neuroepithelial stem cells, neurons and astrocytes", Soc. Neurosci. Abstract Archive, 333.11 (2003) (Abstract Only). Redmond et al., "Calcium Regulation of Dendritic Growth via CaM Kinase IV and CREB-Mediated Transcription", Neuron, 34:999-1010 (2002). Shnaper et al. (2009). Elevated levels of MIC-1/GDF15 in the cerebrospinal fluid patients are associated with glioblastoma and worse outcome. Int. J. Cancer. 125:2624-2630. Goldstein et al., "Lens Epithelium-Derived Growth Factor (LEDGF) in the Retina and Brain", ARVO/IOVS, 41(4):4626-B573 (2000) (Abstract Only). Casiano et al., "Tumor-associated antigen arrays for the serological diagnosis of cancer", Mol. Cell.Proteomics, 5(10):1745-1759 (2006). Bonni et al., "Regulation of Gliogenesis in the Central Nervous System by the JAK-STAT Signaling Pathway", Science, 278:477-483 (1997). Westbrook et al. (2003/0105594) 20030600 Goldstein et al., "Expression and Further Characterization of Alzheimer's Disease Beta-amyloid (Abeta) in Human Aqueous Humor and Lens", Invest. Ophthalmol. Vis. Sci., Abstract No. 3488-B191 (2003). |
| References_xml | – year: 20040500 ident: 2004/0091487 – reference: Rubinson et al., "A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference", Nat. Genet., 33(3):401-406 (2003). – year: 20031000 ident: 2003/0190708 – year: 20040200 ident: 2004/0038348 – year: 20031100 ident: 2003/0215875 – reference: Shnaper et al. (2009). Elevated levels of MIC-1/GDF15 in the cerebrospinal fluid patients are associated with glioblastoma and worse outcome. Int. J. Cancer. 125:2624-2630. – reference: Fatma et al., "Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, by Lens Epithelium-derived Growth Factor to Protect Cells from Oxidative Stress", J. Biol. Chem., 276(52):48899-48907 (2001). – reference: Llano et al., "LEDGF/p75 Determines Cellular Trafficking of Diverse Lentiviral but Not Murine Oncoretroviral Integrase Proteins and Is a Component of Functional Lentiviral Preintegration Complexes", J. Virol., 78(17):9524-9537 (2004). – reference: Daniels et al., "Antinuclear Autoantibodies in Prostate Cancer: Immunity to LEDGF/p75, a Survival Protein Highly Expressed in Prostate Tumors and Cleaved During Apoptosis", Prostate, 62:14-26 (2005). – reference: GenBank GeneID:11168, updated Feb. 3, 2008. – year: 20000500 ident: WO 00/29578 – reference: Maertens et al., "Identification and Characterization of a Functional Nuclear Localization Signal in the HIV-1 Integrase Interactor LEDGF/p75", J. Biol. Chem., 279(32):33421-33429 (2004). – reference: Nakagawa et al., "Regulation of Neurogenesis in Adult Mouse Hippocampus by cAMP and the cAMP Response Element-Binding Protein", J. Neurosci.,22(9):3673-3682 (2002). – reference: Goldstein et al., "Beta-Amyloid Promotes Lens Protein Aggregation and is Associated with Supranuclear Lens Opacification", Invest. Ophthalmol. Vis. Sci., 43 (2002) (Abstract Only). – reference: Goldstein et al., "Alzheimer's Disease β-Amyloid (Aβ) in the Lens: Interactions with AlphaB-Crystallin, Small Heat Schock Proteins, and Redox-Active Metal", Invest. Ophthalmol. Vis. Sci., 45:3507 (2004) (Abstract Only). – reference: Llano et al., "Lens Epithelium-derived Growth Factor/p75 Prevents Proteasomal Degradation of HIV-1 Integrase", J. Biol. Chem., 279(53):55570-55577 (2004). – reference: Fu et al., "Differential Expression of Lens Epithelial-derived Growth Factor (LEDGF) During Neuroepithelial Stem Cell Differentiation in The CNS", ARVO Meeting Abstracts, 45:1719 (May 1, 2004) (Abstract Only). – year: 19910700 ident: 0 438 520 – reference: Fujioka et al., "Activation of cAMP Signaling Facilitates the Morphological Maturation of Newborn Neurons in Adult Hippocampus", J. Neurosci., 24(2):319-328 (2004). – year: 19960900 ident: WO 96/26782 – year: 20050200 ident: WO 2005/015236 – reference: Vanegas et al., "Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering", J. Cell Sci., 118(Pt. 8):1733-1743 (2005). – year: 20040700 ident: 2004/0142333 – reference: Bernier et al., "Characterization of the subventricular zone of the adult human brain: evidence for the involvement of Bcl-2" , Neurosci. Res., 37(1):67-78 (2000). – reference: Ge et al., "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation", Embo J., 17(22):6723-6729 (1998). – year: 19900700 ident: WO 90/07380 – year: 20021000 ident: 2002/0155119 – reference: Johe et al., "Single factors direct the differentiation of stem cells from the fetal and adult central nervous system", Genes Dev., 10:3129-3140 (1996). – year: 20030600 ident: 2003/0105594 – year: 20050100 ident: WO 2005/005601 – reference: Crino et al., "Presence and phosphorylation of transcription factors in developing dendrites", Proc. Natl. Acad. Sci. USA, 95:2313-2318 (1998). – reference: Busschots et al., "The Interaction of LEDGF/p75 with Integrase is Lentivirus-specific and Promotes DNA Binding", J. Biol. Chem., 280(18):17841-17847 (2005). – reference: Goldstein et al., "Lens Epithelium-Derived Growth Factor (LEDGF) in the Retina and Brain", Invest. Ophthalmol. Vis. Sci., 41(4):S870, Abstract No. 4626-B573 (2000). – reference: Walton et al., "CREB Phosphorylation Promotes Nerve Cell Survival", J. Neurochem., 73(5):1836-1842 (1999). – reference: Buc-Caron, M.H., "Neuroepithelial progenitor cells explanted from human fetal brain proliferate and differentiate in vitro", Neurobiol. Dis., 2:37-47 (1995). – reference: Murphy et al., "Morphological plasticity of dendritic spines in central neurons is mediated by activation of cAMP response element binding protein", Proc. Natl. Acad. Sci. USA, 94:1482-1487 (1997). – reference: Goldstein et al., "Lens Epithelium-Derived Growth Factor (LEDGF) in the Retina and Brain", ARVO/IOVS, 41(4):4626-B573 (2000) (Abstract Only). – reference: Pons et al., "Vitronectin regulates Sonic hedgehog activity during cerebellum development through CREB phosphorylation", Development, 128:1481-1492 (2001). – reference: Emiliani et al., "Integrase Mutants Defective for Interaction with LEDGF/p75 Are Impaired in Chromosome Tethering and HIV-1 Replication", J. Biol. Chem., 280(27):25517-25523 (2005). – year: 20040500 ident: 2004/0087505 – reference: Mancini et al., "Lens epithelial-derived growth factor (LEDGF) is differentially expressed and localized in mammalian neuroepithelial stem cells, neurons and astrocytes", Soc. Neurosci. Abstract Archive, 333.11 (2003) (Abstract Only). – reference: Singh et al., "LEDGF Binds to Heat Shock and Stress-Related Element to Activate the Expression of Stress-Related Genes", Biochem. Biophys. Res. Commun., 283:943-955 (2001). – reference: Chylack et al., "Lens epithelium-derived growth factor (LEDGF/p75) expression in fetal and adult human brain", Exp. Eye Res., 79:941-948 (2004). – reference: Monti et al., "NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development", Eur. J. Neurosci., 16:1490-1498 (2002). – year: 20040600 ident: 6750052 – reference: Hussey et al., "Fusion of the NUP98 gene with the LEDGF/p52 gene defines a recurrent acute myeloid leukemia translocation", BMC Genetics, 2:20-23 (2001). – year: 20040300 ident: 6699967 – reference: Dietz et al., "The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies", Biochem. J., 366:491-500 (2002). – reference: GenBank Accession No. AAC25167, Mar. 23, 2001. – reference: Goldstein et al., "Expression and Further Characterization of Alzheimer's Disease Beta-amyloid (Abeta) in Human Aqueous Humor and Lens", Invest. Ophthalmol. Vis. Sci., Abstract No. 3488-B191 (2003). – reference: Giachino et al., "cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb", J. Neurosci., 25(44):10105-10118 (2005). – reference: Lonze et al., "Function and Regulation of CREB Family Transcription Factors in the Nervous System", Neuron, 35:605-623 (2002). – reference: GenBank Accession No. AF098482, Dec. 23, 1998. – year: 20030300 ident: 2003/0059868 – reference: Casiano et al., "Tumor-associated antigen arrays for the serological diagnosis of cancer", Mol. Cell.Proteomics, 5(10):1745-1759 (2006). – reference: Walton et al., "Immediate Early Gene Transcription and Synaptic Modulation", J. Neurosci. Res., 58(1):96-106 (1999). – reference: Redmond et al., "Calcium Regulation of Dendritic Growth via CaM Kinase IV and CREB-Mediated Transcription", Neuron, 34:999-1010 (2002). – reference: Bonni et al., "Regulation of Gliogenesis in the Central Nervous System by the JAK-STAT Signaling Pathway", Science, 278:477-483 (1997). – reference: GenBank Accession No. AAF25870, Jul. 31, 2002. – reference: Singh et al., "Lens Epithemlium-Derived Growth Factor: Increased Resistance to Thermal and Oxidative Stresses", Invest. Ophthalmol. Vis. Sci., 40(7):1444-1451 (1999). – reference: Lepourcelet et al., "Insights into developmental mechanisms and cancers in the mammalian intestine derived from serial analysis of gene expression and study of the hepatoma-derived growth factor (HDGF)", Develop. Dis., 32(2):415-427 (2005). – reference: Mancini et al., "Lens Epithelial-Derived Growth Factor (LEDGF) is Expressed in Neurons Within the Mammalian Central Nervous System", Invest. Ophthalmol. Vis. Sci., 44:E-Abstract 4509 (2003) (Abstract Only). – reference: Maertens et al., "Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75", FASEB J., 19(8):1039-1041 (2005). – reference: Riccio et al., "Mediation by a CREB Family Transcription Factor of NGF-Dependent Survival of Sympathetic Neurons", Science, 286:2358-2361 (1999). – reference: Kirschenbaum et al., "In vitro Neuronal Production and Differentiation by Precursor Cells Derived from the Adult Human Forebrain", Cereb. Cortex, 4(6):576-589 (1994). – reference: Singh et al., "Lens Epithelium-Derived Growth Factor: Effects on Growth and Survival of Lens Epithelial Cells, Keratinocytes, and Fibroblasts", Biochem. Biophys. Res. Commun., 267:373-381 (2000). – reference: Kubo et al., "Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells", Histochem. Cell Biol., 119:289-299 (2003). – reference: GenBank Accession No. AAF25871, Jul. 31, 2002. – reference: Daugaard et al. (2007). Lens epithelium-derived growth factor is an Hsp70-2 regulated guardian of lysosomal stability in human cancer. Cancer. Res. 67(6):2559-2567. – year: 20030100 ident: 2003/0008330 – reference: Eriksson et al., "Neurogenesis in the adult human hippocampus", Nat. Med., 4(11):1313-1317 (1998). |
| Score | 2.789059 |
| Snippet | The invention provides methods for diagnosing tumors in mammals using a reagent that bind to LEDGF/p75 or to a nucleic acid encoding LEDGF/p75. For example,... |
| SourceID | uspatents |
| SourceType | Open Access Repository |
| Title | Compositions and methods for diagnosing tumors using LEDGF/p75 |
| URI | https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8168393 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3BTsMwDLW2CQl2AgFiDFAOXMvapm3ohQuiqpBAO4C02zQ3KRfWVk2r_T52AxMXOCaRHCuJYyd6fga4DQNTckKjV8QY8teN8jDkYmaxKoyOMLhPOVH45TXJ36PnVbwaQb7PhdmSGXkN6WLvett09QCupOvdbbznyJ-ZI7Bi9oFd9Vlv9FKXC64fIVM5hjGdMA6LsnwKhySCQraqs7-cRnYMB8uh9wRGpjqFBza9H4iUoAe8cNWbraC4UWgHeSNHIrp-W7dW9EODKYuzRaPiMxDZ09tj7u0nW3-0DGJZ-99KyXOY0GPeXIAoJZaYGt_HMIgwVaiLTYpSIhYmSRTOYPanmMt_xuZwRK48dFC8K5h0bW-uyV12eDOsxRdOJnZG |
| linkProvider | USPTO |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT8MwDLbGQDxOIEDbeOXAtaxN-lgvXGBVeU09gLRbNbcpF9ZWfYi_j93CxAWOSSTHSuJ8TvTZBriWls44oNFIHJT8deMZKLmYmeMlOrXRmvkcKPyycMM3-3HpLAcQbmJh1mRGRkm61DdtXTZFR66k673feKNP_sw5AnPOPvCZfxSrNEqzKdePUL7agm06UZJNdB6EB7BHQshpy5v6F2wEh7ATdb1HMND5Mdyy8f2QpAQ94UVfv7kW5DmKtCe9EZSIpl0XVS3arsFJi4Np6TknIIL5611obCaL3yumscTmt1rqFIb0nNcjEJnCDH1tmigtG30P02Tlo1KIiXZdD8cw_lPM5J-xK9iN7oP4-WHxdAb7hOuy5-Wdw7CpWn1B2NngZbcsXzjDeTY |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Compositions+and+methods+for+diagnosing+tumors+using+LEDGF%2Fp75&rft.inventor=Goldstein%2C+Lee+E&rft.inventor=Fu%2C+Ling&rft.number=8168393&rft.date=2012-05-01&rft.externalDBID=n%2Fa&rft.externalDocID=08168393 |