Method to be used in fluorescence microscopy

A method using fluorescence microscopy for image evaluation using a laser scanning microscope in which an at least partially spectrally resolved detection of the fluorescence spectrum occurs. Reference spectra are used for spectral demixing. Temporally and/or spectrally variable dyes and/or dye comb...

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Main Authors Wolleschensky, Ralf, Zimmermann, Bernhard, Ankerhold, Richard
Format Patent
LanguageEnglish
Published 30.03.2010
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Abstract A method using fluorescence microscopy for image evaluation using a laser scanning microscope in which an at least partially spectrally resolved detection of the fluorescence spectrum occurs. Reference spectra are used for spectral demixing. Temporally and/or spectrally variable dyes and/or dye combinations are employed for recording of the reference spectra. Finally, the recorded reference spectra are inspected for image evaluation.
AbstractList A method using fluorescence microscopy for image evaluation using a laser scanning microscope in which an at least partially spectrally resolved detection of the fluorescence spectrum occurs. Reference spectra are used for spectral demixing. Temporally and/or spectrally variable dyes and/or dye combinations are employed for recording of the reference spectra. Finally, the recorded reference spectra are inspected for image evaluation.
Author Wolleschensky, Ralf
Ankerhold, Richard
Zimmermann, Bernhard
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References Lansford, et al., "Resolution of Multiple Green Fluorescent Protein Color Variants and Dyes Using Two-Photon Microscopy and Imaging Spectroscopy", Journal of Biomedical Optics, SPIE, vol. 6, No. 3, pp. 311-318, Jul. 2001.
Patterson, et al., "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells", Science, vol. 297, No. 5588, pp. 1873-1877, Sep. 13, 2002 (Abstract Only).
(198 29 657) 19990200
Klimant (6602716) 20030800
(2333153) 19990700
Ando, et al., "An Optical Marker Based on the UV-induced Green-to-Red Photoconversion of a Fluorescent Protein", PNAS, vol. 99, No. 20, pp. 12651-12656, Oct. 1, 2002.
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Patterson et al.; "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells"; Science; Sep. 2002; pp. 1873-1877; vol. 297, No. 5588; National Institutes of Health; USA.
Wolleschensky et al. (7009699) 20060300
(19829657) 19990200
Ando et al.; "An Optical Marker Based on the UV-Induced Green-to-Red Photoconversion of a Fluorescent Protein"; Proceedings of the National Acadamy of Sciences of the United States of America; Sep. 2002; pp. 12651-12656; vol. 99, No. 2; National Academy of Sciences; USA.
Schoeppe et al. (6167173) 20001200
(19900135) 19990800
Lansford et al.; "Resolution of Mulitple Green Fluorescent Protein Color Variants and Dyes using Two-Photon Microscopy and Imaging Spectroscopy"; Journal of Biomedical Optics; Jul. 2001; pp. 311-318; vol. 6, No. 3; SPIE Publications, USA.
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