Neutralizing agent for clostridium bacterial neurotoxins and preparation method thereof
clostridium Disclosed is a neutralizing agent for a bacterial neurotoxin and a preparation method thereof. The neutralizing agent contains a flavonol glycoside (A) or a flavonol glycoside (B). The flavonol glycoside (A) has a flavone skelton which has two hydroxyl groups at the 5 and 7 positions, at...
Saved in:
Main Authors | , , |
---|---|
Format | Patent |
Language | English |
Published |
17.01.2006
|
Online Access | Get full text |
Cover
Loading…
Abstract | clostridium Disclosed is a neutralizing agent for a bacterial neurotoxin and a preparation method thereof. The neutralizing agent contains a flavonol glycoside (A) or a flavonol glycoside (B). The flavonol glycoside (A) has a flavone skelton which has two hydroxyl groups at the 5 and 7 positions, at least one hydroxyl group bonding at either one of the 3′, 4′ and 5′ positions, and an ether linkage forming glycoside at the 3 position with a carbohydrate chain containing a rutinose skelton. The flavonol glycoside (B) has also another ether linkage at the 4′ position with a carbohydrate chain containing rhamnose. The neutralizing agent is prepared by separating the thearubigin fraction of black tea extract by elution with methanol/water solvent using a reverse phase liquid chromatography. A fraction eluted with methanol/water solvent containing 40% methanol and a fraction eluted with methanol/water solvent containing 60% methanol have high neutralizing activity, and at least one of them is collected. |
---|---|
AbstractList | clostridium Disclosed is a neutralizing agent for a bacterial neurotoxin and a preparation method thereof. The neutralizing agent contains a flavonol glycoside (A) or a flavonol glycoside (B). The flavonol glycoside (A) has a flavone skelton which has two hydroxyl groups at the 5 and 7 positions, at least one hydroxyl group bonding at either one of the 3′, 4′ and 5′ positions, and an ether linkage forming glycoside at the 3 position with a carbohydrate chain containing a rutinose skelton. The flavonol glycoside (B) has also another ether linkage at the 4′ position with a carbohydrate chain containing rhamnose. The neutralizing agent is prepared by separating the thearubigin fraction of black tea extract by elution with methanol/water solvent using a reverse phase liquid chromatography. A fraction eluted with methanol/water solvent containing 40% methanol and a fraction eluted with methanol/water solvent containing 60% methanol have high neutralizing activity, and at least one of them is collected. |
Author | Sakane, Iwao Nishimura, Masakazu Sawamura, Shin-ichi |
Author_xml | – sequence: 1 givenname: Shin-ichi surname: Sawamura fullname: Sawamura, Shin-ichi – sequence: 2 givenname: Masakazu surname: Nishimura fullname: Nishimura, Masakazu – sequence: 3 givenname: Iwao surname: Sakane fullname: Sakane, Iwao |
BookMark | eNqNzTsKwkAQgOEttPB1h7mA4AMSrUWxshIsZUwmycBmJszOgnh6FTyA1df88E_DSFRoEm4Xym4Y-cXSArYkDo0aVFGTG9ece3hg5WSMEYSyqeuTJQFKDYPRgIbOKtCTd1qDd2SkzTyMG4yJFj9nAU7H6-G8zGlA_1zSvTX8sir2u3K9KbZ_JG_srz3U |
ContentType | Patent |
CorporateAuthor | Ito En, Ltd |
CorporateAuthor_xml | – name: Ito En, Ltd |
DBID | EFH |
DatabaseName | USPTO Issued Patents |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EFH name: USPTO Issued Patents url: http://www.uspto.gov/patft/index.html sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
ExternalDocumentID | 06987126 |
GroupedDBID | EFH |
ID | FETCH-uspatents_grants_069871263 |
IEDL.DBID | EFH |
IngestDate | Sun Mar 05 22:33:15 EST 2023 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-uspatents_grants_069871263 |
OpenAccessLink | https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6987126 |
ParticipantIDs | uspatents_grants_06987126 |
PatentNumber | 6987126 |
PublicationCentury | 2000 |
PublicationDate | 20060117 |
PublicationDateYYYYMMDD | 2006-01-17 |
PublicationDate_xml | – month: 01 year: 2006 text: 20060117 day: 17 |
PublicationDecade | 2000 |
PublicationYear | 2006 |
References | Eiki Satoh, et al., "Black Tea Extract, Thearubigin Fraction, Counteracts the Effect of Tetanus Toxin in Mice", Exp. Biol. Med., vol. 226, No. 6, 2001, pp. 577-580. A. H. Brantner, et al., Journal of Ethnopharmacology, vol. 66, No. 2, XP-002270981, pp. 175-179, "Quality Assessment of Paliurus Spina-Christi Extracts", 1999. Database Embase 'Online!, EMB-2002439528, XP-002270983, 2000. (2-276562) 19901100 (1 236 724) 20020900 (2-306915) 19901200 Eiki Satoh, et al., "Black Tea Extract, Thearubigin Fraction, Counteract the Effects of Botulinum Neurotoxins in Mice", British Journal of Pharmacology, vol. 132, 2001, pp. 797-798. H. Itokawa, et al., Phytochemistry, vol. 20, No. 10, XP-001179712, pp. 2421-2422, "Flavonol Glycosides from the Flowers of Cucurbita Pepo", 1981. Eiki Satoh, et al., "The Mechanism Underlying the Protective Effect of the Thearubigin Fraction of Black Tea (Camellia Sinensis) Extract Against the Neuromuscular Blocking Action of Botulinum Neurotoxins", Pharmacology & Toxicology, vol. 90, 2002, pp. 199-202. Eiki Satoh, et al., "A Mechanism of the Thearubigin Fraction of Black Tea (Camellia Sinensis) Extract Protecting Against the Effect of Tetanus Toxin", The Journal of Toxicological Sciences, vol. 27, No. 5, 2002, pp. 441-447. (2-304079) 19901200 Y.-Y. Mo, et al., Plant Physiology, vol. 107, No. 2, XP-002270982, pp. 603-612, "Analysis of Sweet Cherry (Prunus Avium L.) Leaves for Plant Signal Molecules that Activate the Syrb Gene Required for Synthesis of the Phytotoxin, Syringomycin, by Pseudomonas Syringae PV Syringae", 1995. (WO 01/40213) 20010600 (0 397 914) 19901100 |
References_xml | – year: 19901100 ident: 2-276562 – year: 20020900 ident: 1 236 724 – year: 19901200 ident: 2-306915 – year: 19901200 ident: 2-304079 – year: 19901100 ident: 0 397 914 – year: 20010600 ident: WO 01/40213 |
Score | 2.6412435 |
Snippet | clostridium Disclosed is a neutralizing agent for a bacterial neurotoxin and a preparation method thereof. The neutralizing agent contains a flavonol glycoside... |
SourceID | uspatents |
SourceType | Open Access Repository |
Title | Neutralizing agent for clostridium bacterial neurotoxins and preparation method thereof |
URI | https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6987126 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fS8MwED62IahPiorzF3nwNdpsSdp3WSmCYw-Kextpk8qgS8raovjXe0l1-KKvCRxHQnL3Hd99B3Abe55OIUpquESAwlhBlZBTOtG5joU0igclpqe5zF7441IsB5DtemE2-Ixojb40d11Tty6QK_F77y-e9uLPXiPQevWBd1s5pRe6vJcIntlEDmGYRJ7aNUuzQ9hHE5iy2bb5FTTSI9hbhNVjGBh7Aq9z04WywidGC6J8SxPBjJEUlfOzM_S625C8105WFQk6k637WNuGINgn9db0Kt3Okn7qM_Gpm3HlKZB09vyQ0Z0Xq7etZ7esom9vp2cwQpRvzoEkwo_m0oybhHGtkjwvy1hzESsTFUrpMYz_NHPxz94lHPxUDVh8BaN225lrjKNtfhMO6QsqhoGq |
link.rule.ids | 230,309,786,808,891,64394 |
linkProvider | USPTO |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS8QwEB7WVXycFBXXZw5eo5vdJu1dt9RX6UFxbyVtUlnoJqUPFH-9SaqLF70mMAwJyTffMPMNwKVv63RyWmDpMUNQCMkxp2yKJyITPmWSe06J6Slm0Yt3P6fzAUSrXpileUa4Mr40V11TtdoVV5rvvb943Is_W41AZdUH3lWpuUhEcc0MeSYTtgbrFmOtiv4sjHZgyxgxQZtqm1-wEe7CRuJW92Ag1T68xrJziYVPgxeI26YmZGJGlJfaTs8Qi26Jsl49mZfIKU22-mOhGmToPqpq2et0a4X6uc_IBm9SFweAwtnzTYRXXqRvta1vScff_k4PYWh4vjwCFFA7nEsQTwbEEzzIsqLwhUd9Lsc552IEoz_NHP-zdwGbyW2YPt7FDyew_ZNCIP4pDNu6k2cGVNvs3J3XF6fGhKQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Neutralizing+agent+for+clostridium+bacterial+neurotoxins+and+preparation+method+thereof&rft.inventor=Sawamura%2C+Shin-ichi&rft.inventor=Nishimura%2C+Masakazu&rft.inventor=Sakane%2C+Iwao&rft.number=6987126&rft.date=2006-01-17&rft.externalDBID=n%2Fa&rft.externalDocID=06987126 |