Culturing encapsulated chondrocytes under reduced oxygen partial pressure to produce cartilage implants
The invention relates to a process for the production of human cartilage implants from, e.g., chondrocytes cultivated in vitro. A process is provided for the production of a human cartilage implant from chondrocytes cultured in vitro, which come as close as possible to the original with respect to t...
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| Main Authors | , , , , |
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| Format | Patent |
| Language | English |
| Published |
04.05.2004
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| Online Access | Get full text |
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| Summary: | The invention relates to a process for the production of human cartilage implants from, e.g., chondrocytes cultivated in vitro.
A process is provided for the production of a human cartilage implant from chondrocytes cultured in vitro, which come as close as possible to the original with respect to their biochemical composition and biomechanical properties. Up to 20% vol. of human serum is used as medium addition in the process. The chondrocytes can be kept in monolayer culture until the 12passage in order firstly to be re-differentiated, incubated under a reduced oxygen partial pressure, and subsequently stimulated to form a three-dimensional cartilage tissue due to aggregation under an oxygen partial pressure of 21%. In an embodiment, chondrocytes in alginate beads are cultured in a nutrient solution, which may contain human serum and one or more chondrogenic growth factors, under an oxygen partial pressure of less than 20 volume %, isolated from the alginate beads by a treatment with a chelating agent, aggregated by centrifugation and cultured under an oxygen partial pressure of: −21 volume %. |
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