Assay methods for nucleic acid in a sample

The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a de...

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Main Authors Heroux, Jeffrey A, Corcoran, Marta L, Rao, Savitha M
Format Patent
LanguageEnglish
Published 21.10.2003
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Abstract The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a detectable species covalently linked. The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a detectable species covalently linked.
AbstractList The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a detectable species covalently linked. The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a detectable species covalently linked.
Author Rao, Savitha M
Corcoran, Marta L
Heroux, Jeffrey A
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References Respess (5599662) 19970200
Eberle et al. (5413906) 19950500
Briggs, Jonathan, et al, Anal. Chem., 1991, 63, 850-859, "Quantitation of DNA and Protein Impurities in Biopharmaceuticals".
Caskey et al. (5364759) 19941100
Wu et al. The ligation amplification reaction (LAR)-amplification of specific DNA sequences using sequential rounds of template-dependent ligation. Genomics , vol. 4, p. 560-569.
Merrick et al. Threshold, a complete system for quantitative analysis of total DNA, protein impurities and relevant proteins, 1992, Biotech Forum Europe, vol. 9(6), pp. 398-403.
Hartley (5043272) 19910800
Kozlowski et al. (6096499) 20000800
Feinberg, Andrew P., et al, Analytical Biochemistry, 132, 6-13 (1983), "A Technique for Radiolabeling DNA Restriction Endonuclease Fragments to High Specific Activity".
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Snippet The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid...
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Title Assay methods for nucleic acid in a sample
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