A controllable Y-shaped DNA structure assisted aptasensor for the simultaneous detection of AFB and OTA based on ARGET ATRP
The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B 1 (A...
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Published in | Journal of materials chemistry. B, Materials for biology and medicine Vol. 12; no. 24; pp. 5861 - 5868 |
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Main Authors | , , , , , |
Format | Journal Article |
Published |
19.06.2024
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Online Access | Get full text |
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Abstract | The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B
1
(AFB
1
) and ochratoxin A (OTA) was developed. In this sensing system, AFB
1
and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, and were used as recognition probes to specifically capture the target. Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB
1
with limits of 426.18 and 79.55 fg mL
−1
for AFB
1
and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
A fluorescent aptasensor was successfully constructed for sensitive AFB
1
and OTA quantification based on a controllable Y-shaped DNA structure with ARGET ATRP. |
---|---|
AbstractList | The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B
1
(AFB
1
) and ochratoxin A (OTA) was developed. In this sensing system, AFB
1
and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, and were used as recognition probes to specifically capture the target. Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB
1
with limits of 426.18 and 79.55 fg mL
−1
for AFB
1
and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
A fluorescent aptasensor was successfully constructed for sensitive AFB
1
and OTA quantification based on a controllable Y-shaped DNA structure with ARGET ATRP. |
Author | Cui, Zhenzhen Yang, Huaixia Miao, Mingsan Jia, Yuzhen Xue, Jinyan Guo, Liang |
AuthorAffiliation | Pharmacy College Henan University of Chinese Medicine Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao |
AuthorAffiliation_xml | – name: Pharmacy College – name: Henan University of Chinese Medicine – name: Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao |
Author_xml | – sequence: 1 givenname: Mingsan surname: Miao fullname: Miao, Mingsan – sequence: 2 givenname: Liang surname: Guo fullname: Guo, Liang – sequence: 3 givenname: Jinyan surname: Xue fullname: Xue, Jinyan – sequence: 4 givenname: Yuzhen surname: Jia fullname: Jia, Yuzhen – sequence: 5 givenname: Zhenzhen surname: Cui fullname: Cui, Zhenzhen – sequence: 6 givenname: Huaixia surname: Yang fullname: Yang, Huaixia |
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Snippet | The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of... |
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Title | A controllable Y-shaped DNA structure assisted aptasensor for the simultaneous detection of AFB and OTA based on ARGET ATRP |
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