Effect of trimethylamine--oxide on the phase separation of aqueous polyethylene glycol-600-Dextran-75 two-phase systems
The emergence of phase separation in both intracellular biomolecular condensates (membrane-less organelles) and in vitro aqueous two-phase systems (ATPS) relies on the formation of immiscible water-based phases/domains. The solvent properties and arrangement of hydrogen bonds within these domains ha...
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Published in | Physical chemistry chemical physics : PCCP Vol. 26; no. 14; pp. 1546 - 1556 |
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Main Authors | , , , , , |
Format | Journal Article |
Published |
03.04.2024
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Abstract | The emergence of phase separation in both intracellular biomolecular condensates (membrane-less organelles) and
in vitro
aqueous two-phase systems (ATPS) relies on the formation of immiscible water-based phases/domains. The solvent properties and arrangement of hydrogen bonds within these domains have been shown to differ and can be modulated with the addition of various inorganic salts and osmolytes. The naturally occuring osmolyte, trimethylamine-
N
-oxide (TMAO), is well established as a biological condensate stabilizer whose presence results in enhanced phase separation of intracellular membrane-less compartments. Here, we show the unique effect of TMAO on the mechanism of phase separation in model PEG-600-Dextran-75 ATPS using dynamic and static light scattering in conjunction with ATR-FTIR and solvatochromic analysis. We observe that the presence of TMAO may enhance or destabilize phase separation depending on the concentration of phase forming components. Additionally, the behavior and density of mesoscopic polymer agglomerates, which arise prior to macroscopic phase separation, are altered by the presence and concentration of TMAO.
Trimethylamine-
N
-oxide enhances or suppresses phase separation in aqueous two-phase systems depending on the composition and concentration of phase forming components. |
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AbstractList | The emergence of phase separation in both intracellular biomolecular condensates (membrane-less organelles) and
in vitro
aqueous two-phase systems (ATPS) relies on the formation of immiscible water-based phases/domains. The solvent properties and arrangement of hydrogen bonds within these domains have been shown to differ and can be modulated with the addition of various inorganic salts and osmolytes. The naturally occuring osmolyte, trimethylamine-
N
-oxide (TMAO), is well established as a biological condensate stabilizer whose presence results in enhanced phase separation of intracellular membrane-less compartments. Here, we show the unique effect of TMAO on the mechanism of phase separation in model PEG-600-Dextran-75 ATPS using dynamic and static light scattering in conjunction with ATR-FTIR and solvatochromic analysis. We observe that the presence of TMAO may enhance or destabilize phase separation depending on the concentration of phase forming components. Additionally, the behavior and density of mesoscopic polymer agglomerates, which arise prior to macroscopic phase separation, are altered by the presence and concentration of TMAO.
Trimethylamine-
N
-oxide enhances or suppresses phase separation in aqueous two-phase systems depending on the composition and concentration of phase forming components. |
Author | Streletzky, Kiril A Zaslavsky, Boris Y Titus, Amber R Herron, Patrick Uversky, Vladimir N Madeira, Pedro P |
AuthorAffiliation | 3615 Superior Ave Department of Chemistry Centro de Investigacao em Materiais Ceramicos e Compositos University of South Florida Cleveland State University Morsani College of Medicine Cleveland Diagnostics Department of Physics Department of Molecular Medicine and Byrd Alzheimer's Research Institute |
AuthorAffiliation_xml | – sequence: 0 name: Cleveland State University – sequence: 0 name: Centro de Investigacao em Materiais Ceramicos e Compositos – sequence: 0 name: Department of Chemistry – sequence: 0 name: Department of Physics – sequence: 0 name: 3615 Superior Ave – sequence: 0 name: Department of Molecular Medicine and Byrd Alzheimer's Research Institute – sequence: 0 name: University of South Florida – sequence: 0 name: Cleveland Diagnostics – sequence: 0 name: Morsani College of Medicine |
Author_xml | – sequence: 1 givenname: Amber R surname: Titus fullname: Titus, Amber R – sequence: 2 givenname: Patrick surname: Herron fullname: Herron, Patrick – sequence: 3 givenname: Kiril A surname: Streletzky fullname: Streletzky, Kiril A – sequence: 4 givenname: Pedro P surname: Madeira fullname: Madeira, Pedro P – sequence: 5 givenname: Vladimir N surname: Uversky fullname: Uversky, Vladimir N – sequence: 6 givenname: Boris Y surname: Zaslavsky fullname: Zaslavsky, Boris Y |
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in vitro
aqueous two-phase systems (ATPS)... |
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Title | Effect of trimethylamine--oxide on the phase separation of aqueous polyethylene glycol-600-Dextran-75 two-phase systems |
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