Naphthalimide based fluorescent organic nanoparticles in selective sensing of Fe and as a diagnostic probe for Fe/Fe transition
Fluorescent organic nanoparticles (FONPs) have attracted considerable attention as a practical and effective platform for sensing and imaging applications. The present article delineates the fabrication of FONPs derived from the naphthalimide based histidine appended amphiphile, NID . The self-assem...
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| Published in | Journal of materials chemistry. B, Materials for biology and medicine Vol. 9; no. 2; pp. 494 - 57 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Published |
21.01.2021
|
| Online Access | Get full text |
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| Summary: | Fluorescent organic nanoparticles (FONPs) have attracted considerable attention as a practical and effective platform for sensing and imaging applications. The present article delineates the fabrication of FONPs derived from the naphthalimide based histidine appended amphiphile,
NID
. The self-assembly of
NID
in 99 vol% water in DMSO led to the formation of FONPs through J-type aggregation. Aggregation-induced emission (AIE) was observed due to the pre-associated excimer of
NID
with bluish green emission at 470 nm along with intramolecular charge transfer (ICT). The emission of
NID
FONPs was utilized for selective sensing of Fe
3+
and bioimaging of Fe
3+
inside mammalian cells. The fluorescence intensity of the FONPs was quenched with the gradual addition of Fe
3+
due to the formation of a 1 : 1 stoichiometric complex with the histidine residue of
NID
. The morphology of the FONPs transformed from spherical to spindle upon the complex formation of
NID
with Fe
3+
. The limit of detection (LOD) of this AIE based turn-off chemosensor for Fe
3+
was found to be 12.5 ± 1.2 μM having high selectivity over other metal ions. On the basis of the very low cytotoxicity and selective sensing of Fe
3+
,
NID
FONPs were successfully employed for bioimaging of Fe
3+
ions through fluorescence quenching within mammalian cells (NIH3T3, B16F10). Considering the varying oxidative stress inside different cells,
NID
FONPs were used for detecting Fe
2+
to Fe
3+
redox state transition selectively inside cancer cells (B16F10) in comparison to non-cancerous cells (NIH3T3). Selective sensing of cancer cells was substantiated by co-culture experiment and flow cytometry. Hence,
NID
FONPs can be a selective diagnostic probe for cancer cells owing to their higher H
2
O
2
content.
Bluish green emitting fluorescent organic nanoparticles (FONPs) with AIE showed selective sensing of Fe
3+
with a LOD of 12.5 ± 1.2 mM and was exploited in bio-imaging and detection of Fe
2+
/Fe
3+
transition inside cancer cells due to their high H
2
O
2
content. |
|---|---|
| Bibliography: | FONPs in NIH3T3 and B16F10 cells, real time imaging NID FONPs included NIH3T3 and B16F10 cells, bright-field and fluorescence microscopic image of co-cultured NIH3T3 and B16F10 cells after 6 h incubation with sensors, characterization data of 99 vol%, emission spectra and photographs of UV irradiated 99 vol% in presence of EDTA, photographs of UV irradiated FONPs in presence of different metal ions, Fe CD spectra of MTT-based % cell viability of 10.1039/d0tb02450c O intensity averaged distribution and correlogram of 99 vol%, stability of sensing and selectivity studies in phosphate buffer medium, emission spectra of FONPs obtained by at 3+ FONPs + Fe Electronic supplementary information (ESI) available: Synthetic scheme of 99 vol% in presence of 2-aminopyridine, citric acid FONPs, emission spectra of different mole fraction of Fe H-NMR 13 99% in DMSO in presence of Fe f NID FONPs in presence and absence of Fe , l See DOI Dopa, EDTA, folic acid and glycine, emission spectra of of equimolar 1 2 at and H w in different ratios of DMSO-water and at comparison of Fe 2+ C-NMR, mass spectrum of |
| ISSN: | 2050-750X 2050-7518 |
| DOI: | 10.1039/d0tb02450c |