A facile method for fabricating a three-dimensional aligned fibrous scaffold for vascular applicationElectronic supplementary information (ESI) available. See DOI: 10.1039/c9ra00661c
Vascular graft replacement remains the optimal treatment option for many vascular diseases despite advances in endovascular surgery. In this study, we proposed the use of surface topographical cues to align and maintain the phenotype of vascular smooth muscle cells (vSMCs) which were reported as one...
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30.04.2019
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Abstract | Vascular graft replacement remains the optimal treatment option for many vascular diseases despite advances in endovascular surgery. In this study, we proposed the use of surface topographical cues to align and maintain the phenotype of vascular smooth muscle cells (vSMCs) which were reported as one of the vital limitations for successful graft replacement. An auxiliary electrospinning setup has been developed to collect circumferentially aligned fibres on a 3D tubular format; this micro-architecture was found to be similar to the tunica media layer of blood vessels. The presence of aligned fibres served as a signaling modality to induce cell alignment and the maintenance of the contractile phenotype. vSMCs cultured on the 3D aligned fibrous substrate were found to exhibit better cell proliferation ability and enhanced cell-shape directionality. The functional expression of the two representative intracellular contractile proteins (
i.e.
α-SMA and MHC) was found to exhibit definitive markers that are orderly organized as microfilament bundles. Collectively, the result suggests a possibility of adapting the 3D aligned tubular scaffold to enhance and regulate cell function along with the additional tunability of scaffold diameter and thicknesses for tailoring to the needs of individual patients or future
ex vivo
studies.
Collection of circumferentially aligned and 3D fibrous scaffold on a newly designed electrospinning auxiliary jig. The aligned fibres served as a signaling modality to induce cell alignment and the maintenance of a contractile phenotype for hSMCs. |
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AbstractList | Vascular graft replacement remains the optimal treatment option for many vascular diseases despite advances in endovascular surgery. In this study, we proposed the use of surface topographical cues to align and maintain the phenotype of vascular smooth muscle cells (vSMCs) which were reported as one of the vital limitations for successful graft replacement. An auxiliary electrospinning setup has been developed to collect circumferentially aligned fibres on a 3D tubular format; this micro-architecture was found to be similar to the tunica media layer of blood vessels. The presence of aligned fibres served as a signaling modality to induce cell alignment and the maintenance of the contractile phenotype. vSMCs cultured on the 3D aligned fibrous substrate were found to exhibit better cell proliferation ability and enhanced cell-shape directionality. The functional expression of the two representative intracellular contractile proteins (
i.e.
α-SMA and MHC) was found to exhibit definitive markers that are orderly organized as microfilament bundles. Collectively, the result suggests a possibility of adapting the 3D aligned tubular scaffold to enhance and regulate cell function along with the additional tunability of scaffold diameter and thicknesses for tailoring to the needs of individual patients or future
ex vivo
studies.
Collection of circumferentially aligned and 3D fibrous scaffold on a newly designed electrospinning auxiliary jig. The aligned fibres served as a signaling modality to induce cell alignment and the maintenance of a contractile phenotype for hSMCs. |
Author | Tay, Bee Yen Chen, Hui Zhi Ong, Yee Oon Cao, Ye Tan, Lay Poh Tran, Le Quan Ngoc Ng, Feng Lin |
AuthorAffiliation | Singapore Institute of Manufacturing Technology School of Materials Science & Engineering Nanyang Technological University |
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Author_xml | – sequence: 1 givenname: Feng Lin surname: Ng fullname: Ng, Feng Lin – sequence: 2 givenname: Yee Oon surname: Ong fullname: Ong, Yee Oon – sequence: 3 givenname: Hui Zhi surname: Chen fullname: Chen, Hui Zhi – sequence: 4 givenname: Le Quan Ngoc surname: Tran fullname: Tran, Le Quan Ngoc – sequence: 5 givenname: Ye surname: Cao fullname: Cao, Ye – sequence: 6 givenname: Bee Yen surname: Tay fullname: Tay, Bee Yen – sequence: 7 givenname: Lay Poh surname: Tan fullname: Tan, Lay Poh |
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References_xml | – issn: 2014 issue: 1175 end-page: p 103-126 publication-title: Lightweight Materials from Biopolymers and Biofibers doi: Xu Yang – issn: 2016 volume-title: Ignace Verpoest, Aart Willem Van Vuure end-page: p 127-155 publication-title: Natural Fiber Composites doi: Le Quan Ngoc Tran – issn: 2015 publication-title: Preparation and characterization of PVDF-PVP-TiO composite hollow fiber membranes for oily wastewater treatment using submerged membrane system doi: Lau Chi Siang Goh Ng Ismail – issn: 2015 publication-title: Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels doi: Agrawal Lee Irvine An Bhuthalingam Singh Low Kai Chua Venkatraman – issn: 2015 publication-title: Interfacial Compatibility and Adhesion in Natural Fiber Composites doi: Tran Fuentes Verpoest Vuure |
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Snippet | Vascular graft replacement remains the optimal treatment option for many vascular diseases despite advances in endovascular surgery. In this study, we proposed... |
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Title | A facile method for fabricating a three-dimensional aligned fibrous scaffold for vascular applicationElectronic supplementary information (ESI) available. See DOI: 10.1039/c9ra00661c |
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