Rapid detection of Group B (GBS) from artificial urine samples based on IFAST and ATP bioluminescence assay: from development to practical challenges during protocol testing in Kenya

• Published in: Analyst (London) Vol. 144; no. 23; pp. 6889 - 6897
• Main Authors: Ngamsom, Bongkot, Wandera, Ernest Apondi, Iles, Alexander, Kimani, Racheal, Muregi, Francis, Gitaka, Jesse, Pamme, Nicole
• Format: Journal Article
• Published: 18.11.2019

We report the rapid detection (20 min) of Streptococcus agalactiae , Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS using an adenosine triphosphate (ATP) bioluminesc...