Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational designElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ob00376a
The 62nd residue of glycosyltransferase UGT BL 1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH t...
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| Main Authors | , , , , |
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| Format | Journal Article |
| Language | English |
| Published |
04.04.2018
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| Online Access | Get full text |
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| Abstract | The 62nd residue of glycosyltransferase UGT
BL
1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol.
Via
semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product.
Conduct structure-guided modification on the "hotspot" of glycosyltransferase UGT
BL
1 to significantly adjust its regioselectivity toward polydatin production. |
|---|---|
| AbstractList | The 62nd residue of glycosyltransferase UGT
BL
1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol.
Via
semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product.
Conduct structure-guided modification on the "hotspot" of glycosyltransferase UGT
BL
1 to significantly adjust its regioselectivity toward polydatin production. |
| Author | Dong, Wenxin Chu, Jianlin Fan, Bo Chen, Tianyi He, Bingfang |
| AuthorAffiliation | School of Pharmaceutical Sciences Nanjing Tech University Jiangsu National Synergetic Innovation Center for Advanced Materials College of Biotechnology and Pharmaceutical Engineering |
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| Author_xml | – sequence: 1 givenname: Bo surname: Fan fullname: Fan, Bo – sequence: 2 givenname: Wenxin surname: Dong fullname: Dong, Wenxin – sequence: 3 givenname: Tianyi surname: Chen fullname: Chen, Tianyi – sequence: 4 givenname: Jianlin surname: Chu fullname: Chu, Jianlin – sequence: 5 givenname: Bingfang surname: He fullname: He, Bingfang |
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| DOI | 10.1039/c8ob00376a |
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| Snippet | The 62nd residue of glycosyltransferase UGT
BL
1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol.
Via
semi-rational design including... |
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| Title | Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational designElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ob00376a |
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