Tuning calcium biosensors with a single-site mutation: structural dynamics insights from femtosecond Raman spectroscopyElectronic supplementary information (ESI) available: Further discussion on the significance and perspective of this work, Fig. S1-S4 and Tables S1, S2, and additional references. See DOI: 10.1039/c6cp08821j

• Main Authors: Tachibana, Sean R, Tang, Longteng, Wang, Yanli, Zhu, Liangdong, Liu, Weimin, Fang, Chong
• Format: Journal Article
• Language: English
• Published: 08.03.2017

Fluorescent protein biosensors are popular reporters for biological processes and life sciences, but their fundamental working mechanisms remain unclear. To characterize the functional fluorescence events on their native timescales, we implemented wavelength-tunable femtosecond stimulated Raman spec...