Anomalous pressure effects on the photoreaction of a light-sensor protein from Synechocystis, PixD (Slr1694), and the compressibility change of its intermediatesElectronic supplementary information (ESI) available. See DOI: 10.1039/c6cp05091c
SyPixD (Slr1694) is a blue-light receptor that contains a BLUF (blue-light sensor using a flavin chromophore) domain for the function of phototaxis. The key reaction of this protein is a light-induced conformational change and subsequent dissociation reaction from the decamer to the dimer. In this s...
Saved in:
Main Authors | , , , , , , |
---|---|
Format | Journal Article |
Published |
21.09.2016
|
Online Access | Get full text |
Cover
Loading…
Abstract | SyPixD (Slr1694) is a blue-light receptor that contains a BLUF (blue-light sensor using a flavin chromophore) domain for the function of phototaxis. The key reaction of this protein is a light-induced conformational change and subsequent dissociation reaction from the decamer to the dimer. In this study, anomalous effects of pressure on this reaction were discovered, and changes in the compressibility of its short-lived intermediates were investigated. While the absorption spectra of the dark and light states are not sensitive to pressure, the formation yield of the first intermediate decreases with pressure to about 40% at 150 MPa. Upon blue-light illumination with a sufficiently strong intensity, the transient grating signal, which represents the dissociation of the SyPixD decamer, was observed at 0.1 MPa, and the signal intensity significantly decreased with increasing pressure. This behavior shows that the dissociation of the decamer from the second intermediate state is suppressed by pressure. However, while the decamer undergoes no dissociation upon excitation of one monomer unit at 0.1 MPa, dissociation is gradually induced with increasing pressure. For solving this strange behavior, the compressibility changes of the intermediates were measured as a function of pressure at weak light intensity. Interestingly, the compressibility change was negative at low pressure, but became positive with increasing pressure. Because the compressibility is related to the volume fluctuation, this observation suggests that the driving force for this reaction is fluctuation of the protein. The relationship between the cavities at the interfaces of the monomer units and the reactivity was also discussed.
High pressure enhances the dissociation yield of SyPixD, and this pressure effect was explained by pressure dependent fluctuation of intermediates. |
---|---|
AbstractList | SyPixD (Slr1694) is a blue-light receptor that contains a BLUF (blue-light sensor using a flavin chromophore) domain for the function of phototaxis. The key reaction of this protein is a light-induced conformational change and subsequent dissociation reaction from the decamer to the dimer. In this study, anomalous effects of pressure on this reaction were discovered, and changes in the compressibility of its short-lived intermediates were investigated. While the absorption spectra of the dark and light states are not sensitive to pressure, the formation yield of the first intermediate decreases with pressure to about 40% at 150 MPa. Upon blue-light illumination with a sufficiently strong intensity, the transient grating signal, which represents the dissociation of the SyPixD decamer, was observed at 0.1 MPa, and the signal intensity significantly decreased with increasing pressure. This behavior shows that the dissociation of the decamer from the second intermediate state is suppressed by pressure. However, while the decamer undergoes no dissociation upon excitation of one monomer unit at 0.1 MPa, dissociation is gradually induced with increasing pressure. For solving this strange behavior, the compressibility changes of the intermediates were measured as a function of pressure at weak light intensity. Interestingly, the compressibility change was negative at low pressure, but became positive with increasing pressure. Because the compressibility is related to the volume fluctuation, this observation suggests that the driving force for this reaction is fluctuation of the protein. The relationship between the cavities at the interfaces of the monomer units and the reactivity was also discussed.
High pressure enhances the dissociation yield of SyPixD, and this pressure effect was explained by pressure dependent fluctuation of intermediates. |
Author | Tokutomi, Satoru Nakajima, Tsubasa Terazima, Masahide Okajima, Koji Kuroi, Kunisato Nakasone, Yusuke Ikeuchi, Masahiko |
AuthorAffiliation | Department of Chemistry Kyoto University Department of Biological Science Osaka Prefecture University Department of Life Sciences (Biology) Research Institute for Advanced Science and Technology The University of Tokyo Graduate School of Science Graduate School of Arts and Sciences |
AuthorAffiliation_xml | – name: Graduate School of Arts and Sciences – name: Department of Chemistry – name: Kyoto University – name: Research Institute for Advanced Science and Technology – name: The University of Tokyo – name: Graduate School of Science – name: Osaka Prefecture University – name: Department of Biological Science – name: Department of Life Sciences (Biology) |
Author_xml | – sequence: 1 givenname: Tsubasa surname: Nakajima fullname: Nakajima, Tsubasa – sequence: 2 givenname: Kunisato surname: Kuroi fullname: Kuroi, Kunisato – sequence: 3 givenname: Yusuke surname: Nakasone fullname: Nakasone, Yusuke – sequence: 4 givenname: Koji surname: Okajima fullname: Okajima, Koji – sequence: 5 givenname: Masahiko surname: Ikeuchi fullname: Ikeuchi, Masahiko – sequence: 6 givenname: Satoru surname: Tokutomi fullname: Tokutomi, Satoru – sequence: 7 givenname: Masahide surname: Terazima fullname: Terazima, Masahide |
BookMark | eNqFkEFLAzEQhYNUsFUv3oU5ttDWhG1X15vYij0prPeSprPdSJJZMqm4f9tf4FpEDx48zTDz5nuPGYheoIBCXCg5VTIrrkxuGjmXhTJHoq9meTYp5M2s99Nf5ydiwPwqpVRzlfXFx10grx3tGZqIzPuIgFWFJjFQgFQjNDUliqhNst2EKtDg7K5OE8bAFLs7SmgDVJE8lG1AU5NpOVkew7N9X8CwdFHlxWw0Bh22B6Yhf7CzG-tsasHUOuzwC247YxsSRo9bqxPy0nVhIgVrgPdN49BjSDq2naqi6PUh1XBZrkag37R1euNwCiUiLJ5Wt_D3M2fiuNKO8fy7norLh-XL_eMkslk30foOvv6VZ__tPwHSoXt0 |
ContentType | Journal Article |
DOI | 10.1039/c6cp05091c |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 1463-9084 |
EndPage | 25925 |
ExternalDocumentID | c6cp05091c |
GroupedDBID | -JG 0-7 1TJ 705 70J 70~ 7~J AAEMU ABGFH ACLDK ADSRN AEFDR AFVBQ AGSTE AUDPV BSQNT C6K EE0 EF- GNO H~N IDZ J3G J3I R7B R7C RCNCU RPMJG RRC RSCEA SKA SKF SLH VH6 |
ID | FETCH-rsc_primary_c6cp05091c3 |
ISSN | 1463-9076 |
IngestDate | Mon Jan 28 17:10:42 EST 2019 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 37 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-rsc_primary_c6cp05091c3 |
Notes | Electronic supplementary information (ESI) available. See DOI 10.1039/c6cp05091c |
PageCount | 11 |
ParticipantIDs | rsc_primary_c6cp05091c |
ProviderPackageCode | J3I ACLDK RRC 7~J AEFDR 70~ VH6 GNO RCNCU SLH 70J EE0 RSCEA AFVBQ C6K H~N 0-7 IDZ RPMJG 1TJ SKA -JG AGSTE AUDPV EF- BSQNT SKF ADSRN ABGFH 705 R7B AAEMU J3G R7C |
PublicationCentury | 2000 |
PublicationDate | 20160921 |
PublicationDateYYYYMMDD | 2016-09-21 |
PublicationDate_xml | – month: 9 year: 2016 text: 20160921 day: 21 |
PublicationDecade | 2010 |
PublicationYear | 2016 |
References_xml | – issn: 2007 end-page: p 255-338 publication-title: Adv. Photochem. doi: Terazima |
SSID | ssj0001513 |
Score | 4.4834256 |
Snippet | SyPixD (Slr1694) is a blue-light receptor that contains a BLUF (blue-light sensor using a flavin chromophore) domain for the function of phototaxis. The key... |
SourceID | rsc |
SourceType | Publisher |
StartPage | 25915 |
Title | Anomalous pressure effects on the photoreaction of a light-sensor protein from Synechocystis, PixD (Slr1694), and the compressibility change of its intermediatesElectronic supplementary information (ESI) available. See DOI: 10.1039/c6cp05091c |
Volume | 18 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1LT9tAEMdXaTi0F9QXaqFUc2glEJjiXT9ibghSkdKWQ1KJntB62QpDsCM7rpp-6B74BJ19-JEmSG0vVrKWN4nnl92Z9fxnCXkj3JjJnqDOvoyY44WUO5yF0unR2EN_PGRGSPvpc3Dyxftw7p93Or9aWUvlNN4TP5fqSv7HqtiGdlUq2X-wbN0pNuBrtC8e0cJ4_CsbY-x-y8cqiVWns6pnAXV-hklfnFxlGFRLuyG41kKOdemQAsPXLN_RZRqS1KhMhrNU4mgoZvi3N_X7kx_HygUdjnM3iLy3NKqSPVXfKhtdZ9Hq_NqZ1RCbEraFrkORa10KOrP9ZrOdQm0jalLWc6U5rNWT6oP6w4FapuDfeTJWmq49HMvkzvHZQC9caO2-KlwqAjFRFWVcUa9l8xt-ndxqT3hUlDg319PNaZlnJmVBP7mfZu1rMNrQK7pfy6K8qRk_azo7za6T9sqIG6g0DiO3toO5FzAHg39barvdZralW5wByqoGTTWe-5FRm1rnAN8bnfbCzLP0989X8m5OPiArNIx8v0tWDvujwcfad0D_ixk9nPnmVUFdFr1rrkY3KK-2p9Fu0OgxWbXxCxwaGJ-QjkyfkodH1baBz8hdDSVUUIKFErIUEByYgxKyb8ChDSVYKEFBCXNQ7oJCErYskNu7gDDqPv-AEQyMqnOEEe6BEeZghBaMsIUobkMDIiCIgCAewOKdek423_dHRycO3q-LiSnfctGcZmukmyJnLwj4gsX-Jcd4RAj0cXmPMm8_Fr04lC69pPQlWVvex_p9JzbIowbKV6Q7zUu5iQ7sNH5trf4bVe-mdw |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Royal Society of Chemistry |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Anomalous+pressure+effects+on+the+photoreaction+of+a+light-sensor+protein+from+Synechocystis%2C+PixD+%28Slr1694%29%2C+and+the+compressibility+change+of+its+intermediatesElectronic+supplementary+information+%28ESI%29+available.+See+DOI%3A+10.1039%2Fc6cp05091c&rft.au=Nakajima%2C+Tsubasa&rft.au=Kuroi%2C+Kunisato&rft.au=Nakasone%2C+Yusuke&rft.au=Okajima%2C+Koji&rft.date=2016-09-21&rft.issn=1463-9076&rft.eissn=1463-9084&rft.volume=18&rft.issue=37&rft.spage=25915&rft.epage=25925&rft_id=info:doi/10.1039%2Fc6cp05091c&rft.externalDocID=c6cp05091c |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1463-9076&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1463-9076&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1463-9076&client=summon |