Rapid synthesis of highly luminescent and stable Au20 nanoclusters for active tumor-targeted imaging in vitro and in vivoElectronic supplementary information (ESI) available: Additional figures and tables. See DOI: 10.1039/c3nr05269a
Rapid synthesis of protein-stabilized Au 20 nanoclusters (Au 20 NCs) with high fluorescence quantum yield (QY) up to ∼15% is successfully achieved by manipulating the reaction kinetics. The as-obtained Au 20 NCs, identified by mass spectrometry, have an average size of 2.6 nm, with strong fluorescen...
Saved in:
Main Authors | , , , , , |
---|---|
Format | Journal Article |
Language | English |
Published |
30.01.2014
|
Online Access | Get full text |
Cover
Loading…
Abstract | Rapid synthesis of protein-stabilized Au
20
nanoclusters (Au
20
NCs) with high fluorescence quantum yield (QY) up to ∼15% is successfully achieved by manipulating the reaction kinetics. The as-obtained Au
20
NCs, identified by mass spectrometry, have an average size of 2.6 nm, with strong fluorescence emission at 620 nm (2.00 eV) upon excitation at either 370 nm (3.35 eV) or 470 nm (2.64 eV). The advantages of the as-obtained Au
20
NCs, including small sizes, high fluorescence QY, excellent photostability, non-toxicity, and good stability in biological media, make them ideal candidates as good luminescent probes for optical imaging
in vitro
and
in vivo
. Our results demonstrate that the uptake of Au
20
NCs by both cancer cells and tumor-bearing nude mice can be improved by receptor-mediated internalization, compared with that by passive targeting. Because of their selective accumulation at the tumor sites, the Au
20
NC probes can be used as potential indicators for cancer diagnosis. This work not only provides a new understanding of the rapid synthesis of highly luminescent Au
20
NCs but also demonstrates that the functionalized-Au
20
NCs are excellent probes for active tumor-targeted imaging
in vitro
and
in vivo
.
Receptor-mediated uptake of nanoprobes for tumor-targeting
in vitro
and
in vivo
is systematically studied using newly prepared luminescent Au
20
nanoclusters. |
---|---|
AbstractList | Rapid synthesis of protein-stabilized Au
20
nanoclusters (Au
20
NCs) with high fluorescence quantum yield (QY) up to ∼15% is successfully achieved by manipulating the reaction kinetics. The as-obtained Au
20
NCs, identified by mass spectrometry, have an average size of 2.6 nm, with strong fluorescence emission at 620 nm (2.00 eV) upon excitation at either 370 nm (3.35 eV) or 470 nm (2.64 eV). The advantages of the as-obtained Au
20
NCs, including small sizes, high fluorescence QY, excellent photostability, non-toxicity, and good stability in biological media, make them ideal candidates as good luminescent probes for optical imaging
in vitro
and
in vivo
. Our results demonstrate that the uptake of Au
20
NCs by both cancer cells and tumor-bearing nude mice can be improved by receptor-mediated internalization, compared with that by passive targeting. Because of their selective accumulation at the tumor sites, the Au
20
NC probes can be used as potential indicators for cancer diagnosis. This work not only provides a new understanding of the rapid synthesis of highly luminescent Au
20
NCs but also demonstrates that the functionalized-Au
20
NCs are excellent probes for active tumor-targeted imaging
in vitro
and
in vivo
.
Receptor-mediated uptake of nanoprobes for tumor-targeting
in vitro
and
in vivo
is systematically studied using newly prepared luminescent Au
20
nanoclusters. |
Author | Wang, Yi Huang, Cheng Zhi Xiong, Zu Hong Zhao, Ning Wei Zhang, Pu Yang, Xiao Xi |
AuthorAffiliation | Education Ministry Key Laboratory on Luminescence and Real-Time Analysis School of Physical Science and Technology Southwest University Shimadzu (China) Co Life Science & Clinical Medicine Department College of Pharmaceutical Sciences School of Chemistry and Chemical Engineering |
AuthorAffiliation_xml | – name: Shimadzu (China) Co – name: School of Physical Science and Technology – name: School of Chemistry and Chemical Engineering – name: College of Pharmaceutical Sciences – name: Life Science & Clinical Medicine Department – name: Southwest University – name: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis |
Author_xml | – sequence: 1 givenname: Pu surname: Zhang fullname: Zhang, Pu – sequence: 2 givenname: Xiao Xi surname: Yang fullname: Yang, Xiao Xi – sequence: 3 givenname: Yi surname: Wang fullname: Wang, Yi – sequence: 4 givenname: Ning Wei surname: Zhao fullname: Zhao, Ning Wei – sequence: 5 givenname: Zu Hong surname: Xiong fullname: Xiong, Zu Hong – sequence: 6 givenname: Cheng Zhi surname: Huang fullname: Huang, Cheng Zhi |
BookMark | eNqFkE9Lw0AQxRepYKtevAvjTQ-t22yNtLdiK_YkWO9l3EzSkc1u2N0E8pH9FqZB9CDoaf78hvceMxID6ywJcTGVk6lU81utrJd3STrHIzFM5EyOlbpPBt99OjsRoxDepUznKlVD8fGCFWcQWhv3FDiAy2HPxd60YOqSLQVNNgLa7ibimyFY1okEi9ZpU4dIPkDuPKCO3BDEunR-HNEXFCkDLrFgWwBbaDh61-v0Q-PWhnS3sqwh1FVlqOyM0Lcd7wRLjOwsXK-3mxvABtkczBewzDI-EDSQc1F7Cr1mHy1MYEsEq-fNAn4_5Ewc52gCnX_VU3H5uH59eBr7oHeV76L6dvdzrv7nV3_xXZXl6hNQ-IQd |
ContentType | Journal Article |
DOI | 10.1039/c3nr05269a |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EISSN | 2040-3372 |
EndPage | 2269 |
ExternalDocumentID | c3nr05269a |
ID | FETCH-rsc_primary_c3nr05269a3 |
ISSN | 2040-3364 |
IngestDate | Thu May 19 04:17:41 EDT 2016 Thu May 30 17:32:39 EDT 2019 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-rsc_primary_c3nr05269a3 |
Notes | 10.1039/c3nr05269a Electronic supplementary information (ESI) available: Additional figures and tables. See DOI |
PageCount | 9 |
ParticipantIDs | rsc_primary_c3nr05269a |
PublicationCentury | 2000 |
PublicationDate | 20140130 |
PublicationDateYYYYMMDD | 2014-01-30 |
PublicationDate_xml | – month: 1 year: 2014 text: 20140130 day: 30 |
PublicationDecade | 2010 |
PublicationYear | 2014 |
SSID | ssj0069363 |
Score | 4.2597914 |
Snippet | Rapid synthesis of protein-stabilized Au
20
nanoclusters (Au
20
NCs) with high fluorescence quantum yield (QY) up to ∼15% is successfully achieved by... |
SourceID | rsc |
SourceType | Enrichment Source Publisher |
StartPage | 2261 |
Title | Rapid synthesis of highly luminescent and stable Au20 nanoclusters for active tumor-targeted imaging in vitro and in vivoElectronic supplementary information (ESI) available: Additional figures and tables. See DOI: 10.1039/c3nr05269a |
Volume | 6 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1Lj9MwEMet0r3AAS2PFbvsojlwAEUpbV5t9lagsAviIVi05VTl4a4sdZMqTSrBR-Sb8C2YsZ3EFUECLlFjK1aS-XU8dsZ_M_Y4xj6MJ25qB0iv7Y0cbk_ocyFGFuOJx7GTiGmg-O59cPbFezP3573eDyNrqSrjQfK9c13J_1gVy9CutEr2HyzbNIoF-Bvti0e0MB7_ysaforVISXQAozgtLELyw6tvFrocymdP6gxyjAFpidS0coZWFmV5sqpIIWGjkiilz7PK6jovbJUajmGouFYbGInM2oqyyLVOE55s81m7ec6GtgVVKegFrSFsVkNS7Dr7fE7TDtE2Eiu6ATkNmaZCT0AuxVWFw32VxUn1mwE6L269_HAuZyrkYn1SKk3crCDJmLDpRJqJ7o9V47h0yVxEOR7aTwWq-Kswrs3VvwAf75ILc-ZjRNky9Ucc6SAdyoZ0XaWCPuBm2XjHwwcGyJ7prR0lBK97fjwNO3uVzkfdVeluK2-wPWcc-n6f7U3fPn99WccFQejKff2au67Fct3wWXs1hjhFvfWMDHEu9tltPTaBqQLtDuvx7C67ZShW3mM_JXLQIAf5EhRyYCAHaFBQyAEhByZygISAQg52kQONHIgMJHKyHXliIgc7yIGBHDxB4J5Cg9sptLCBhk22qWEDhA0QtlP4_RXdZyevZhcvzmx8UYu10mRZtNXuAetnecYfkB6BE8WeP0yjUeqFySTyI2c89GPuJYEfpsEhO-hu45AddVcs1uny6E9XPWQ3W0yPWb8sKn6CIWsZP9Is_AKxWKEV |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Royal Society of Chemistry |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Rapid+synthesis+of+highly+luminescent+and+stable+Au20+nanoclusters+for+active+tumor-targeted+imaging+in+vitro+and+in+vivoElectronic+supplementary+information+%28ESI%29+available%3A+Additional+figures+and+tables.+See+DOI%3A+10.1039%2Fc3nr05269a&rft.au=Zhang%2C+Pu&rft.au=Yang%2C+Xiao+Xi&rft.au=Wang%2C+Yi&rft.au=Zhao%2C+Ning+Wei&rft.date=2014-01-30&rft.issn=2040-3364&rft.eissn=2040-3372&rft.volume=6&rft.issue=4&rft.spage=2261&rft.epage=2269&rft_id=info:doi/10.1039%2Fc3nr05269a&rft.externalDocID=c3nr05269a |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2040-3364&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2040-3364&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2040-3364&client=summon |