The structure and activation of substrate water molecules in the S2 state of photosystem II studied by hyperfine sublevel correlation spectroscopyThis study is supported by the Photosynthetic Systems Program, Office of Basic Energy Sciences, United States Department of Energy (DE-FG02-07ER15903).Electronic supplementary information (ESI) available. See DOI: 10.1039/c2ee21210b
The water-splitting protein, photosystem II, catalyzes the light-driven oxidation of water to dioxygen. The solar water oxidation reaction takes place at the catalytic center, referred to as the oxygen-evolving complex, of photosystem II. During the catalytic cycle, the oxygen-evolving complex cycle...
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Format | Journal Article |
Language | English |
Published |
20.06.2012
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Abstract | The water-splitting protein, photosystem II, catalyzes the light-driven oxidation of water to dioxygen. The solar water oxidation reaction takes place at the catalytic center, referred to as the oxygen-evolving complex, of photosystem II. During the catalytic cycle, the oxygen-evolving complex cycles through five distinct intermediate states, S
0
-S
4
. In this study, we trap the oxygen-evolving complex in the S
2
intermediate state by low temperature illumination of photosystem II isolated from three different species,
Thermosynechococcus vulcanus
, the PsbB variant of
Synechocystis
PCC 6803 and spinach. We apply two-dimensional hyperfine sublevel correlation spectroscopy to detect weak magnetic interactions between the paramagnetic tetra-nuclear manganese cluster of the S
2
state of the OEC and the surrounding protons. We identify five groups of protons that are interacting with the tetra-nuclear manganese cluster. From the values of hyperfine interactions and using the recently reported 1.9 Å resolution X-ray structure of the OEC in the S
1
state [Umena
et al.
,
Nature
, 2011,
473
, 55], we discuss the assignments of the five groups of protons and draw important conclusions on the structure of the oxygen-evolving complex in the S
2
state. In addition, we conclude that the structure of OEC is nearly identical in photosystem II from
Thermosynechococcus vulcanus
, the PsbB variant of
Synechocystis
PCC 6803 and spinach.
A high-resolution two-dimensional
1
H HYSCORE spectroscopy study of the structure and activation of substrate water molecules in the S
2
state of photosystem II. |
---|---|
AbstractList | The water-splitting protein, photosystem II, catalyzes the light-driven oxidation of water to dioxygen. The solar water oxidation reaction takes place at the catalytic center, referred to as the oxygen-evolving complex, of photosystem II. During the catalytic cycle, the oxygen-evolving complex cycles through five distinct intermediate states, S
0
-S
4
. In this study, we trap the oxygen-evolving complex in the S
2
intermediate state by low temperature illumination of photosystem II isolated from three different species,
Thermosynechococcus vulcanus
, the PsbB variant of
Synechocystis
PCC 6803 and spinach. We apply two-dimensional hyperfine sublevel correlation spectroscopy to detect weak magnetic interactions between the paramagnetic tetra-nuclear manganese cluster of the S
2
state of the OEC and the surrounding protons. We identify five groups of protons that are interacting with the tetra-nuclear manganese cluster. From the values of hyperfine interactions and using the recently reported 1.9 Å resolution X-ray structure of the OEC in the S
1
state [Umena
et al.
,
Nature
, 2011,
473
, 55], we discuss the assignments of the five groups of protons and draw important conclusions on the structure of the oxygen-evolving complex in the S
2
state. In addition, we conclude that the structure of OEC is nearly identical in photosystem II from
Thermosynechococcus vulcanus
, the PsbB variant of
Synechocystis
PCC 6803 and spinach.
A high-resolution two-dimensional
1
H HYSCORE spectroscopy study of the structure and activation of substrate water molecules in the S
2
state of photosystem II. |
Author | Chatterjee, Ruchira Koua, Faisal H. M Lakshmi, K. V Shen, Jian-Ren Milikisiyants, Sergey Coates, Christopher S |
AuthorAffiliation | Division of Bioscience Rensselaer Polytechnic Institute Graduate School of Natural Science and Technology and Faculty of Science Department of Chemistry and Chemical Biology and The Baruch '60 Center for Biochemical Solar Energy Research Okayama University |
AuthorAffiliation_xml | – name: Graduate School of Natural Science and Technology and Faculty of Science – name: Division of Bioscience – name: Department of Chemistry and Chemical Biology and The Baruch '60 Center for Biochemical Solar Energy Research – name: Rensselaer Polytechnic Institute – name: Okayama University |
Author_xml | – sequence: 1 givenname: Sergey surname: Milikisiyants fullname: Milikisiyants, Sergey – sequence: 2 givenname: Ruchira surname: Chatterjee fullname: Chatterjee, Ruchira – sequence: 3 givenname: Christopher S surname: Coates fullname: Coates, Christopher S – sequence: 4 givenname: Faisal H. M surname: Koua fullname: Koua, Faisal H. M – sequence: 5 givenname: Jian-Ren surname: Shen fullname: Shen, Jian-Ren – sequence: 6 givenname: K. V surname: Lakshmi fullname: Lakshmi, K. V |
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Notes | This study is supported by the Photosynthetic Systems Program, Office of Basic Energy Sciences, United States Department of Energy (DE-FG02-07ER15903). 10.1039/c2ee21210b Electronic supplementary information (ESI) available. See DOI |
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Title | The structure and activation of substrate water molecules in the S2 state of photosystem II studied by hyperfine sublevel correlation spectroscopyThis study is supported by the Photosynthetic Systems Program, Office of Basic Energy Sciences, United States Department of Energy (DE-FG02-07ER15903).Electronic supplementary information (ESI) available. See DOI: 10.1039/c2ee21210b |
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