Evaluation of the In Vitro Cytotoxicity of Crosslinked Biomaterials
This study evaluated the in vitro cytotoxicity of poly(propylene fumarate) (PPF). PPF is an aliphatic biodegradable polymer that has been well characterized for use in bone tissue engineering scaffolds. Four different cell types, human mesenchymal stem cells (hMSC), fibroblasts (L929), pre-osteoblas...
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Published in | Biomacromolecules Vol. 14; no. 5; pp. 1321 - 1329 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
29.04.2013
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Online Access | Get full text |
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Abstract | This study evaluated the
in vitro
cytotoxicity of poly(propylene fumarate) (PPF). PPF is an aliphatic biodegradable polymer that has been well characterized for use in bone tissue engineering scaffolds. Four different cell types, human mesenchymal stem cells (hMSC), fibroblasts (L929), pre-osteoblasts (MC3T3), and canine mesenchymal stem cells (cMSC), were used to evaluate the cytotoxicity of PPF. These cell types represent the tissues that PPF would interact with
in vivo
as a bone tissue scaffold. The sol fraction of the PPF films was measured and then utilized to estimate crosslinking density. Cytotoxicity was evaluated using XTT assay and fluorescence imaging. Results showed that PPF supported similar cell metabolic activities of hMSC, L929, MC3T3 and cMSC compared to the non-cytotoxic control, high density polyethylene (HDPE) and were statistically different than those cultured with the cytotoxic control, a polyurethane film containing 0.1% zinc diethyldithiocarbamate (ZCF). Results showed differing cellular responses to ZCF, the cytotoxic control. The L929 cells had the lowest cell metabolic activity levels after exposure to ZCF compared to the cell metabolic activity levels of the MC3T3, hMSC or cMSC cells. Qualitative verification of the results using fluorescence imaging demonstrated no change in cell morphology, vacuolization, or detachment when cultured with PPF compared to HDPE or blank media cultures. Overall the cytotoxicity response of the cells to PPF was demonstrated to be similar to the cytotoxic response of cells to known non-cytotoxic materials (HDPE). |
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AbstractList | This study evaluated the
in vitro
cytotoxicity of poly(propylene fumarate) (PPF). PPF is an aliphatic biodegradable polymer that has been well characterized for use in bone tissue engineering scaffolds. Four different cell types, human mesenchymal stem cells (hMSC), fibroblasts (L929), pre-osteoblasts (MC3T3), and canine mesenchymal stem cells (cMSC), were used to evaluate the cytotoxicity of PPF. These cell types represent the tissues that PPF would interact with
in vivo
as a bone tissue scaffold. The sol fraction of the PPF films was measured and then utilized to estimate crosslinking density. Cytotoxicity was evaluated using XTT assay and fluorescence imaging. Results showed that PPF supported similar cell metabolic activities of hMSC, L929, MC3T3 and cMSC compared to the non-cytotoxic control, high density polyethylene (HDPE) and were statistically different than those cultured with the cytotoxic control, a polyurethane film containing 0.1% zinc diethyldithiocarbamate (ZCF). Results showed differing cellular responses to ZCF, the cytotoxic control. The L929 cells had the lowest cell metabolic activity levels after exposure to ZCF compared to the cell metabolic activity levels of the MC3T3, hMSC or cMSC cells. Qualitative verification of the results using fluorescence imaging demonstrated no change in cell morphology, vacuolization, or detachment when cultured with PPF compared to HDPE or blank media cultures. Overall the cytotoxicity response of the cells to PPF was demonstrated to be similar to the cytotoxic response of cells to known non-cytotoxic materials (HDPE). |
Author | Fisher, John P. Thompson, Joshua A. Dean, David Etheridge, Julie M. Wang, Martha O. Vorwald, Charlotte E. |
AuthorAffiliation | 2 Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH 1 Fischell Department of Bioengineering, University of Maryland, College Park, MD |
AuthorAffiliation_xml | – name: 1 Fischell Department of Bioengineering, University of Maryland, College Park, MD – name: 2 Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH |
Author_xml | – sequence: 1 givenname: Martha O. surname: Wang fullname: Wang, Martha O. organization: Fischell Department of Bioengineering, University of Maryland, College Park, MD Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH – sequence: 2 givenname: Julie M. surname: Etheridge fullname: Etheridge, Julie M. organization: Fischell Department of Bioengineering, University of Maryland, College Park, MD Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH – sequence: 3 givenname: Joshua A. surname: Thompson fullname: Thompson, Joshua A. organization: Fischell Department of Bioengineering, University of Maryland, College Park, MD Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH – sequence: 4 givenname: Charlotte E. surname: Vorwald fullname: Vorwald, Charlotte E. organization: Fischell Department of Bioengineering, University of Maryland, College Park, MD Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH – sequence: 5 givenname: David surname: Dean fullname: Dean, David organization: Fischell Department of Bioengineering, University of Maryland, College Park, MD Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH – sequence: 6 givenname: John P. surname: Fisher fullname: Fisher, John P. organization: Fischell Department of Bioengineering, University of Maryland, College Park, MD Department of Neurological Surgery, Case Western Reserve University, Cleveland, OH |
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Snippet | This study evaluated the
in vitro
cytotoxicity of poly(propylene fumarate) (PPF). PPF is an aliphatic biodegradable polymer that has been well characterized... |
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Title | Evaluation of the In Vitro Cytotoxicity of Crosslinked Biomaterials |
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