Aneuploidy and polyploidy underlie adaptive evolution of yeast cells deprived of a conserved cytokinesis motor
The ability to evolve is a fundamental feature of biological systems, but the mechanisms underlying this capacity and the evolutionary dynamics of conserved core processes remain elusive. We show here that yeast cells deleted of MYO1 , encoding the only myosin-II normally required for cytokinesis, r...
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Published in | Cell Vol. 135; no. 5; pp. 879 - 893 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
28.11.2008
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Online Access | Get full text |
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Abstract | The ability to evolve is a fundamental feature of biological systems, but the mechanisms underlying this capacity and the evolutionary dynamics of conserved core processes remain elusive. We show here that yeast cells deleted of
MYO1
, encoding the only myosin-II normally required for cytokinesis, rapidly evolved divergent pathways to restore growth and cytokinesis. The evolved cytokinesis phenotypes correlated with specific changes in the transcriptome. Polyploidy and aneuploidy were common genetic alterations in the best evolved strains, and aneuploidy could account for gene expression changes at levels both correlated with and well beyond chromosome stoichiometry. The phenotypic effect of aneuploidy could be recapitulated with increased copy numbers of specific regulatory genes in
myo1Δ
cells. These results demonstrate the evolvability of even a well-conserved process and suggest that changes in chromosome stoichiometry provide a source of heritable variation driving the emergence of adaptive phenotypes when the cell division machinery is strongly perturbed. |
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AbstractList | The ability to evolve is a fundamental feature of biological systems, but the mechanisms underlying this capacity and the evolutionary dynamics of conserved core processes remain elusive. We show here that yeast cells deleted of
MYO1
, encoding the only myosin-II normally required for cytokinesis, rapidly evolved divergent pathways to restore growth and cytokinesis. The evolved cytokinesis phenotypes correlated with specific changes in the transcriptome. Polyploidy and aneuploidy were common genetic alterations in the best evolved strains, and aneuploidy could account for gene expression changes at levels both correlated with and well beyond chromosome stoichiometry. The phenotypic effect of aneuploidy could be recapitulated with increased copy numbers of specific regulatory genes in
myo1Δ
cells. These results demonstrate the evolvability of even a well-conserved process and suggest that changes in chromosome stoichiometry provide a source of heritable variation driving the emergence of adaptive phenotypes when the cell division machinery is strongly perturbed. |
Author | Li, Rong Staehling-Hampton, Karen Rancati, Giulia Walton, Kendra Seidel, Chris W. Pavelka, Norman Perera, Anoja Allen, Rhonda Fleharty, Brian Noll, Aaron |
AuthorAffiliation | 1 Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A |
AuthorAffiliation_xml | – name: 1 Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A |
Author_xml | – sequence: 1 givenname: Giulia surname: Rancati fullname: Rancati, Giulia organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 2 givenname: Norman surname: Pavelka fullname: Pavelka, Norman organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 3 givenname: Brian surname: Fleharty fullname: Fleharty, Brian organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 4 givenname: Aaron surname: Noll fullname: Noll, Aaron organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 5 givenname: Rhonda surname: Allen fullname: Allen, Rhonda organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 6 givenname: Kendra surname: Walton fullname: Walton, Kendra organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 7 givenname: Anoja surname: Perera fullname: Perera, Anoja organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 8 givenname: Karen surname: Staehling-Hampton fullname: Staehling-Hampton, Karen organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 9 givenname: Chris W. surname: Seidel fullname: Seidel, Chris W. organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A – sequence: 10 givenname: Rong surname: Li fullname: Li, Rong organization: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110, U.S.A |
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Title | Aneuploidy and polyploidy underlie adaptive evolution of yeast cells deprived of a conserved cytokinesis motor |
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