An Enzyme-Linked Immunosorbent Assay (ELISA) for in Vitro Pollen Growth Based on Binding of a Monoclonal Antibody to the Pollen Tube Surface 1

• Published in: Plant physiology (Bethesda) Vol. 84; no. 3; pp. 851 - 855
• Main Authors: Harris, Philip J., Freed, Katy, Anderson, Marilyn A., Weinhandl, Jodie A., Clarke, Adrienne E.
• Format: Journal Article
• Language: English
• Published: 01.07.1987
• Subjects: Development and Growth Regulation

An indirect method of quantifying in vitro pollen growth has been developed. This is based on the finding that a monoclonal antibody (PCBC3), which has a primary specificity for α- l -arabinofuranosyl residues, binds to the surface of in vitro grown pollen tubes of the ornamental tobacco, Nicotiana...