Ecotoxicological assessment of UV filters benzophenone-3 and TiO 2 nanoparticles, isolated and in a mixture, in developing zebrafish ( Danio rerio )

• Published in: Journal of toxicology and environmental health. Part A p. 1
• Main Authors: Moreira Morais, Jéssyca, da Silva Brito, Rafaella, Saiki, Patrícia, Cirqueira Dias, Felipe, de Oliveira Neto, Jerônimo Raimundo, da Cunha, Luiz Carlos, Lopes Rocha, Thiago, Bailão, Elisa Flávia Luiz Cardoso
• Format: Journal Article
• Language: English
• Published: England 05.06.2024
• Subjects: sunscreen; Danio rerio; titanium dioxide nanoparticles; UV filters; emerging pollutants; oxybenzone; Active pharmaceutical ingredients
• ISSN: 1528-7394

The increasing use of UV filters, such as benzophenone-3 (BP-3) and titanium dioxide nanoparticles (TiO NPs), has raised concerns regarding their ecotoxicological effects on the aquatic environment. The aim of the present study was to examine the embryo-larval toxicity attributed to BP-3 or TiO NPs, either alone or in a mixture, utilizing zebrafish ( ) as a model after exposure to environmentally relevant concentrations of these compounds. Zebrafish embryos were exposed to BP-3 (10, 100, or 1000 ng/L) or TiO NPs (1000 ng/L) alone or in a mixture (BP-3 10, 100, or 1000 ng/L plus 1000 ng/L of TiO NPs) under static conditions for 144 hr. After exposure, BP-3 levels were determined by high-performance liquid chromatography (HPLC). BP-3 levels increased in the presence of TiO NPs, indicating that the BP-3 degradation decreased in the presence of the NPs. In addition, in the presence of zebrafish, BP-3 levels in water decreased, indicating that zebrafish embryos and larvae might absorb BP-3. Data demonstrated that, in general, environmentally relevant concentrations of BP-3 and TiO NPs, either alone or in a mixture, did not significantly induce changes in heart and spontaneous contractions frequencies, levels of reactive oxygen species (ROS), morphological and morphometric parameters as well as mortality rates during 144 hr exposure. However, the groups exposed to TiO NPs alone and in a mixture with BP-3 at 10 ng/L exhibited an earlier significant hatching rate than the controls. Altogether, the data indicates that a potential ecotoxicological impact on the aquatic environment exists.