Structural Basis for coronaviral main proteases Inhibition by a 3CL Protease Inhibitor- GC376

• Published in: Journal of molecular biology p. 168474
• Main Authors: Lin, Cheng, Zhu, Zhimin, Jiang, Haihai, Zou, Xiaofang, Zeng, Xiangyi, Wang, Jie, Zeng, Pei, Li, Wenwen, Zhou, Xuelan, Zhang, Jin, Wang, Qisheng, Li, Jian
• Format: Journal Article
• Language: English
• Published: Netherlands 02.02.2024
• Subjects: mutation; crystal; protein; suppressant; virus

The main protease (M ) of coronaviruses participates in viral replication, serving as a hot target for drug design. GC376 is able to effectively inhibit the activity of M , which is due to nucleophilic addition of GC376 by binding covalently with Cys145 in M active site. Here, we used fluorescence r...