Identification and expression analysis of the YABBY gene family in strawberry

• Published in: Sheng wu gong cheng xue bao = Chinese journal of biotechnology Vol. 40; no. 1; p. 104
• Main Authors: Yu, Tingting, Shen, Shurong, Xu, Yiling, Wang, Xinyu, Yu, Yao, Ma, Bojun, Chen, Xifeng
• Format: Journal Article
• Language: Chinese
• Published: China 25.01.2024
• Subjects: Arabidopsis; Biological Assay; Cold Temperature; Computational Biology; Fragaria - genetics; expression pattern; YABBY gene family; subcellular localization; strawberry; sequence analysis

YABBY proteins are important transcription factors that regulate morphogenesis and organ development in plants. In order to study the YABBY of strawberry, bioinformatic technique were used to identify the gene families in (diploid) and × (octoploid), and then analyze the sequence characters, phylogeny and collinearity of the family members. The RNA-seq data and the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) technique were used to assay the expression patterns of the family members. A green fluorescent protein (GFP) was fused with FvYABBYs and transiently expressed in tobacco leaf cells for the subcellular localization. As the results, six genes and 26 genes were identified from . and .× , respectively. The genes were grouped into five clades, and five family members were orthologous with genes of . In . , all of the s were basically not expressed not expressed in root and receptacle, while , , and were highly expressed in leaf, shoot, flower and achene. In .× , , , and were express