Genomic Features of an MDR Escherichia coli ST5506 Harboring an IncHI2/In 229 / bla CTX-M-2 Array Isolated from a Migratory Black Skimmer
Migratory birds have contributed to the dissemination of multidrug-resistant (MDR) bacteria across the continents. A CTX-M-2-producing was isolated from a black skimmer ( ) in Southeast Brazil. The whole genome was sequenced using the Illumina NextSeq platform and de novo assembled by CLC. Bioinform...
Saved in:
Published in | Pathogens (Basel) Vol. 13; no. 1 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
09.01.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Migratory birds have contributed to the dissemination of multidrug-resistant (MDR) bacteria across the continents. A CTX-M-2-producing
was isolated from a black skimmer (
) in Southeast Brazil. The whole genome was sequenced using the Illumina NextSeq platform and de novo assembled by CLC. Bioinformatic analyses were carried out using tools from the Center for Genomic Epidemiology. The genome size was estimated at 4.9 Mb, with 4790 coding sequences. A wide resistome was detected, with genes encoding resistance to several clinically significant antimicrobials, heavy metals, and biocides. The
gene was inserted in an In
class 1 integron inside a ∆Tn
transposon located in an IncHI2/ST2 plasmid. The strain was assigned to ST5506, CH type
, serotype O8:K87, and phylogroup B1. Virulence genes associated with survival in acid conditions, increased serum survival, and adherence were also identified. These data highlight the role of migratory seabirds as reservoirs and carriers of antimicrobial resistance determinants and can help to elucidate the antimicrobial resistance dynamics under a One Health perspective. |
---|---|
AbstractList | Migratory birds have contributed to the dissemination of multidrug-resistant (MDR) bacteria across the continents. A CTX-M-2-producing
was isolated from a black skimmer (
) in Southeast Brazil. The whole genome was sequenced using the Illumina NextSeq platform and de novo assembled by CLC. Bioinformatic analyses were carried out using tools from the Center for Genomic Epidemiology. The genome size was estimated at 4.9 Mb, with 4790 coding sequences. A wide resistome was detected, with genes encoding resistance to several clinically significant antimicrobials, heavy metals, and biocides. The
gene was inserted in an In
class 1 integron inside a ∆Tn
transposon located in an IncHI2/ST2 plasmid. The strain was assigned to ST5506, CH type
, serotype O8:K87, and phylogroup B1. Virulence genes associated with survival in acid conditions, increased serum survival, and adherence were also identified. These data highlight the role of migratory seabirds as reservoirs and carriers of antimicrobial resistance determinants and can help to elucidate the antimicrobial resistance dynamics under a One Health perspective. |
Author | Dutra, Gustavo H P Lincopan, Nilton Fernandes, Miriam R Moura, Quézia Sellera, Fábio P Cardoso, Brenda Nascimento, Cristiane L |
Author_xml | – sequence: 1 givenname: Quézia surname: Moura fullname: Moura, Quézia organization: Postgraduate Program in Infectious Diseases, Federal University of Espírito Santo, Vitória 29047-105, Brazil – sequence: 2 givenname: Miriam R orcidid: 0000-0001-6570-0946 surname: Fernandes fullname: Fernandes, Miriam R organization: Laboratory of Integrative Cancer Immunology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-9760, USA – sequence: 3 givenname: Fábio P orcidid: 0000-0002-4725-0125 surname: Sellera fullname: Sellera, Fábio P organization: School of Veterinary Medicine, Metropolitan University of Santos, Santos 11045-002, Brazil – sequence: 4 givenname: Brenda surname: Cardoso fullname: Cardoso, Brenda organization: One Health Brazilian Resistance Project (OneBR), São Paulo 05508-000, Brazil – sequence: 5 givenname: Cristiane L surname: Nascimento fullname: Nascimento, Cristiane L organization: Veterinary Unit of Santos Aquarium, Santos 11030-600, Brazil – sequence: 6 givenname: Gustavo H P surname: Dutra fullname: Dutra, Gustavo H P organization: Veterinary Unit of Santos Aquarium, Santos 11030-600, Brazil – sequence: 7 givenname: Nilton orcidid: 0000-0003-0161-5800 surname: Lincopan fullname: Lincopan, Nilton organization: Department of Clinical Analysis, Faculty of Pharmacy, University of São Paulo, São Paulo 05508-000, Brazil |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38251370$$D View this record in MEDLINE/PubMed |
BookMark | eNqFjs1OwkAUhScGI6i8grkv0DBMU1qXimC76Ea6cEduh1sYmR9ypyz6CL61kmjizrM550u-xbkVIx88XYmJkvkikcU8H_3ZYzGN8UN-p5AXvhHjtFDZPM3lRHy-kg_OaFgT9memCKED9FC_vMEq6gOx0QeDoIM1sGmyTC6gRG4DG7-_iJXXZaVmlQelHmEGrUVYNu9JnSh4YsYBqhgs9rSDjoMDhNrsGfvAAzxb1EfYHI1zxPfiukMbafrTd-JhvWqWZXI6t4522xMbhzxsf7-n_wpfL5JQLA |
ContentType | Journal Article |
DBID | NPM |
DatabaseName | PubMed |
DatabaseTitle | PubMed |
DatabaseTitleList | PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 2076-0817 |
ExternalDocumentID | 38251370 |
Genre | Journal Article |
GroupedDBID | 53G 5VS 8FE 8FH AADQD AAHBH ADBBV AFKRA AFZYC ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BBNVY BCNDV BENPR BHPHI CCPQU DIK GROUPED_DOAJ HCIFZ HYE IAO IHR KQ8 LK8 M48 M7P MODMG M~E NPM OK1 PIMPY PROAC RIG RPM |
ID | FETCH-pubmed_primary_382513703 |
ISSN | 2076-0817 |
IngestDate | Sun Jun 23 00:36:09 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | seabirds heavy metals whole-genome sequencing ESBL biocides wildlife |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-pubmed_primary_382513703 |
ORCID | 0000-0003-0161-5800 0000-0002-4725-0125 0000-0001-6570-0946 |
PMID | 38251370 |
ParticipantIDs | pubmed_primary_38251370 |
PublicationCentury | 2000 |
PublicationDate | 2024-Jan-09 |
PublicationDateYYYYMMDD | 2024-01-09 |
PublicationDate_xml | – month: 01 year: 2024 text: 2024-Jan-09 day: 09 |
PublicationDecade | 2020 |
PublicationPlace | Switzerland |
PublicationPlace_xml | – name: Switzerland |
PublicationTitle | Pathogens (Basel) |
PublicationTitleAlternate | Pathogens |
PublicationYear | 2024 |
SSID | ssj0000800817 |
Score | 4.580878 |
Snippet | Migratory birds have contributed to the dissemination of multidrug-resistant (MDR) bacteria across the continents. A CTX-M-2-producing
was isolated from a... |
SourceID | pubmed |
SourceType | Index Database |
Title | Genomic Features of an MDR Escherichia coli ST5506 Harboring an IncHI2/In 229 / bla CTX-M-2 Array Isolated from a Migratory Black Skimmer |
URI | https://www.ncbi.nlm.nih.gov/pubmed/38251370 |
Volume | 13 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1bS8MwFMfDFBRfxPtdzoNvoWrbrFsfdTo3YeJlwt4k6Vqpl1bm9qD4BfzWnpO0XRkK6ksY6dIu-_9ITk56Thjb8wPb9QLPtqRSoSUcIS1V71PhIQ2-H6pIZ_u88Fq34rxX7VUqH-XokqHaD96_jSv5j6pYh7pSlOwflC1uihX4GfXFEhXG8lcan4U6qJiTHTcamPSxtPlycs1PX0mNmN5k5qh1jKYfrhI8CtpRaRaZSINDq02pUdsJd8g94DS5epK80e1ZHcvhR4OBfONt7IQkw1SHokjeie-zvXnt_eM3jzF5v8tm7iXalSn-bO3SPcaJ8qnkcehg17TJejUy2_TvcTE35G5t4wOP8T98Hr_SeEObDKZpU7e0VZyOA9QaCHv6mhpiw9zVkHk0HKE9Gv544HMOa56FpoqZiMNv6vKR250ktCT6y7NW3aXYXNccTjKRWTu_NMWmXLtaWo4_ZJZ0XR_YXDx6js3mTSYWIdoY6S6w-WwVAUcGiUVWCZMlNmPOFX1bZp8ZGJCDAWkEMgEEA0pgAIEBBgwowKAvGjAO2gkgFnAACAVkUICGAnIogKAACQUUoKGADIoVtts87TZalunH3YtJbnKX99BdZdNJmoTrDHBVLoJaX_ZF1Bd2LfAjUY0iZddrsi58Wd1gaz_cZPPHK1tsbiz9NpseDkbhDpp3Q7WrdfgCIDVOxA |
link.rule.ids | 315,786,790 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genomic+Features+of+an+MDR+Escherichia+coli+ST5506+Harboring+an+IncHI2%2FIn+229+%2F+bla+CTX-M-2+Array+Isolated+from+a+Migratory+Black+Skimmer&rft.jtitle=Pathogens+%28Basel%29&rft.au=Moura%2C+Qu%C3%A9zia&rft.au=Fernandes%2C+Miriam+R&rft.au=Sellera%2C+F%C3%A1bio+P&rft.au=Cardoso%2C+Brenda&rft.date=2024-01-09&rft.issn=2076-0817&rft.eissn=2076-0817&rft.volume=13&rft.issue=1&rft_id=info%3Apmid%2F38251370&rft.externalDocID=38251370 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2076-0817&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2076-0817&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2076-0817&client=summon |