Genome-wide CRISPRi Screen in Human iNeurons to Identify Novel Focal Cortical Dysplasia Genes

• Published in: bioRxiv : the preprint server for biology
• Main Authors: Tidball, Andrew M, Luo, Jinghui, Walker, J Clayton, Takla, Taylor N, Carvill, Gemma L, Parent, Jack M
• Format: Journal Article
• Language: English
• Published: United States 13.12.2023
• Subjects: epilepsy; focal cortical dysplasia; iNeuron; CRISPRi screen; mTOR; Biological sciences; Neuroscience/Genetics

Focal cortical dysplasia (FCD) is a common cause of focal epilepsy that typically results from brain mosaic mutations in the mTOR cell signaling pathway. To identify new FCD genes, we developed an CRISPRi screen in human neurons and used FACS enrichment based on the FCD biomarker, phosphorylated S6...