Improved Protocol for Reproducible Human Cortical Organoids Reveals Early Alterations in Metabolism with MAPT Mutations

• Published in: bioRxiv : the preprint server for biology
• Main Authors: Bertucci, Taylor, Bowles, Kathryn R, Lotz, Steven, Qi, Le, Stevens, Katherine, Goderie, Susan K, Borden, Susan, Oja, Laura Maria, Lane, Keith, Lotz, Ryan, Lotz, Hailey, Chowdhury, Rebecca, Joy, Shona, Arduini, Brigitte L, Butler, David C, Miller, Michael, Baron, Heide, Sandhof, Carl Alexander, Silva, M Catarina, Haggarty, Stephen J, Karch, Celeste M, Geschwind, Daniel H, Goate, Alison M, Temple, Sally
• Format: Journal Article
• Language: English
• Published: United States 11.07.2023

Cerebral cortical-enriched organoids derived from human pluripotent stem cells (hPSCs) are valuable models for studying neurodevelopment, disease mechanisms, and therapeutic development. However, recognized limitations include the high variability of organoids across hPSC donor lines and experimenta...