High throughput PRIME editing screens identify functional DNA variants in the human genome

• Published in: bioRxiv : the preprint server for biology
• Main Authors: Ren, Xingjie, Yang, Han, Nierenberg, Jovia L, Sun, Yifan, Chen, Jiawen, Beaman, Cooper, Pham, Thu, Nobuhara, Mai, Takagi, Maya Asami, Narayan, Vivek, Li, Yun, Ziv, Elad, Shen, Yin
• Format: Journal Article
• Language: English
• Published: United States 12.07.2023

Despite tremendous progress in detecting DNA variants associated with human disease, interpreting their functional impact in a high-throughput and base-pair resolution manner remains challenging. Here, we develop a novel pooled prime editing screen method, PRIME, which can be applied to characterize...