Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn 2+ -releasing mechanism

• Published in: Journal of structural biology Vol. 215; no. 1; p. 107926
• Main Authors: Zhang, Senfeng, Fu, Chunting, Luo, Yongbo, Xie, Qingrong, Xu, Tong, Sun, Ziyi, Su, Zhaoming, Zhou, Xiaoming
• Format: Journal Article
• Language: English
• Published: United States 01.03.2023
• Subjects: Cryoelectron Microscopy; Eukaryota; Humans; Hydrogen-Ion Concentration; Protons; Zinc; Membrane protein; SLC30; ZnT8; CDF; Single-particle reconstruction; Protonation; Transport cycle; Cryo-EM structure; Conformational change

Zinc transporter 8 (ZnT8) is mainly expressed in pancreatic islet β cells and is responsible for H -coupled uptake (antiport) of Zn into the lumen of insulin secretory granules. Structures of human ZnT8 and its prokaryotic homolog YiiP have provided structural basis for constructing a plausible tran...