Advances of CRISPR/Cas9 activation system

Gene editing technology has been a hotspot in the field of biotechnology. CRISPR/Cas systems are efficient gene editing tools because of its specificity, simplicity and flexibility, these features enabled the rapid application of CRISPR/Cas systems in a variety of organisms. Moreover, the combinatio...

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Bibliographic Details
Published inSheng wu gong cheng xue bao = Chinese journal of biotechnology Vol. 38; no. 8; p. 2713
Main Authors Ding, Xiao, Pan, Zhuanxia, Yang, Liuliu, Luo, Xiaoli, Jiang, Nan, Zhu, Mengjie, Wu, Cuicui, Lan, Gang, Li, Pengbo
Format Journal Article
LanguageChinese
Published China 25.08.2022
Subjects
Biotechnology
CRISPR-Cas Systems - genetics
Gene Editing
Phenotype
RNA, Guide, CRISPR-Cas Systems - genetics
RNA, Guide, CRISPR-Cas Systems - metabolism
genome editing
transcription activating element
CRISPR/Cas
guide RNA modification
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Summary:Gene editing technology has been a hotspot in the field of biotechnology. CRISPR/Cas systems are efficient gene editing tools because of its specificity, simplicity and flexibility, these features enabled the rapid application of CRISPR/Cas systems in a variety of organisms. Moreover, the combination of transcriptional activator with dead Cas protein can achieve specific regulation of gene expression at the transcription level, which has made important contributions to the development of biotechnology in medical and agriculture. Overexpression of foreign genes is a common method to verify gene function and regulation. However, due to the limitation of vector capacity, it is difficult to achieve overexpression of multiple genes. CRISPR/Cas9 activation system can regulate the expression of multiple genes under the guidance of different guide RNAs to verify gene functions at the regulatory level. This review summarizes the composition of the CRISPR/Cas9 activation system and different activation strategies, and
ISSN:1872-2075