Determination of Vancomycin and Norvancomycin Residues in Milk by Automated on-Line Solid-Phase Extraction Combined with Liquid Chromatography-High Resolution Mass Spectrometry

• Published in: Journal of AOAC International
• Main Authors: Wang, Hong, Wang, Hong-Peng, Chen, Min-Na, Ai, Lian-Feng, Liang, Shu-Xuan, Zhang, Yan
• Format: Journal Article
• Language: English
• Published: England 18.03.2022
• ISSN: 1944-7922

vancomycin and norvancomycin, as potent antibacterial retention drugs, were illegally acted on food animals, which directly affected the quality and safety of animal-derived food, and even harmed human health. A fast analysis method, which was adopted to detect residues of vancomycin and norvancomycin in milk, was implemented on a chromatographic system containing on-line solid-phase extraction (SPE) device that combined with high resolution mass spectrometer (HRMS). First the analytes added to blank milk sample were extracted with water (containing 0.1% trifluoroacetic acid (TFA)) - acetonitrile (ACN) (8:2, v/v), then purified and enriched on a C18-XL column, whereafter eluted from the purification column onto the analytical column (Shiseido CAPCELL PAK ADME column) for chromatographic separation prior to hybrid quadrupole-Orbitrap (Q-Orbitrap) detection. The results showed that the Limit of detection (LOD) for each analyte and the limit of quantitation (LOQ) were 0.15 μg/kg and 0.5 μg/kg, respectively. The correlation coefficient(s) of vancomycin and norvancomycin ranged from 0-200 ng/mL were greater than 0.9983. These validations reflected that it was suitable for the established method to rapidly analyze vancomycin and norvancomycin residues in milk. The method for detecting vancomycin and norvancomycin residues in milk by on-line SPE combined with LC-HRMS. On-line SPE technology realized automation, and the application of HRMS greatly improved the reliability of qualitative and quantitative analyses. The developed method is fast, simple and reliable, each methodological index can meet requirements of trace analyses of vancomycin and norvancomycin in milk.