A fast and effective method for dissecting parasitic spores: myxozoans as an example

Disassembling the parasitic spores and acquiring the main subunits is a prerequisite for deep understanding of the basic biology of parasites. Herein we present a fast and efficient method to dissect the myxospores in a few steps, which mainly involved sonication, sucrose density gradient and Percol...

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Bibliographic Details
Published inJournal of experimental biology
Main Authors Guo, Qingxiang, Liu, Yang, Zhai, Yanhua, Gu, Zemao
Format Journal Article
LanguageEnglish
Published England 01.01.2020
Subjects
Myxozoans
Parasites
Dissection
Spores
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Summary:Disassembling the parasitic spores and acquiring the main subunits is a prerequisite for deep understanding of the basic biology of parasites. Herein we present a fast and efficient method to dissect the myxospores in a few steps, which mainly involved sonication, sucrose density gradient and Percoll density gradient. We tested our method on three myxozoans species and demonstrated this method allows the dismembering of myxospores, isolation of intact and clean nematocysts and shell valves within 2h by low-cost. This new tool will facilitate subsequent analyses and enable a better understanding of the ecological and evolutionary significance of parasitic spores.
ISSN:1477-9145