The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation

• Published in: Journal of cell science
• Main Authors: Galeano Niño, Jorge Luis, Tay, Szun S, Tearle, Jacqueline L E, Xie, Jianling, Govendir, Matt A, Kempe, Daryan, Mazalo, Jessica, Drew, Alexander P, Colakoglu, Feyza, Kummerfeld, Sarah K, Proud, Christopher G, Biro, Maté
• Format: Journal Article
• Language: English
• Published: England 01.01.2020
• Subjects: Flow cytometry; Translation; T cell activation; Actin; Lifeact; Live imaging

It has become increasingly evident that T cell functions are subject to translational control in addition to transcriptional regulation. Live imaging of CD8+ T cells isolated from the Lifeact-EGFP mouse reveals that they exhibit a gain in fluorescence intensity following engagement of cognate tumour...